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Detection of Mycoplasma hyopneumoniae in lungs and nasal swabs of pigs by nested PCR Arq. Bras. Med. Vet. Zootec.
Silva,F.M.F.; Castro,L.A.; Silva Júnior,A.; Moraes,M.P.; Moreira,M.A.S.; Almeida,M.R..
Fifty-four samples were collected from growing and finishing pigs for the molecular diagnosis of enzootic porcine pneumonia. Nineteen lung fragments were obtained from pigs that showed signs of respiratory disease and 35 nasal swabs were obtained from clinically healthy pigs. For the detection of the bacterial genome in the samples, the nested PCR technique was used to amplify a fragment of 706bp. This fragment was subsequently cloned and sequenced. The sequence of obtained nucleotides was compared with six other sequences of Mycoplasma hyopneumoniae and 11 sequences of other bacteria available in the Genbank. To measure the sensitivity of the nested PCR, serial dilutions (10-1 to 10-15) of cloned fragments were conducted based on the concentration of...
Tipo: Info:eu-repo/semantics/report Palavras-chave: Swine; Enzootic pneumonia; Mycoplasma hyopneumoniae; Nested PCR.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352009000100021
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