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	Provedor de dados BABT (1) Autor Araújo,Nathália Kelly de (1) Assis,Cristiane Fernandes de (1) Macedo,Gorete Ribeiro de (1) Pagnoncelli,Maria Giovana Binder (1) Rodrigues,Sueli (1) Silva,Nayane Macêdo Portela da (1) Mais... Palavra-chave Chitosanase (1) Chitosanase production (1) Crude enzymes and TLC (1) Paenibacillus ehimensis (1) Tipo do documento Info:eu-repo/semantics/article (1) Ano 2010 (1) País Brazil (1) Idioma Inglês (1)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 1 Primeira ... 1 ... Última Chitosanase production by Paenibacillus ehimensis and its application for chitosan hydrolysis BABT Pagnoncelli,Maria Giovana Binder; Araújo,Nathália Kelly de; Silva,Nayane Macêdo Portela da; Assis,Cristiane Fernandes de; Rodrigues,Sueli; Macedo,Gorete Ribeiro de. The chitosanase production by Paenibacillus ehimensis was studied in submerged cultures and the chitosan hydrolysis was evaluated by using these enzymes without purification. The bacterium produced inducibles enzymes after 12 h of growth in a culture medium containing 0.2% (w/v) of soluble chitosan as carbon source. The enzyme production was strongly repressed by the presence of glucose. The production started as soon as the available sugars finished in the culture medium. The maximum level of chitosanase activity was 500 U.L-1 at 36°C after 36 h incubation. The crude enzyme was optimally active at pH 6.0 and 55°C and in these conditions, the enzyme presented good stability (6 days). The enzyme without purification was used to hydrolyze the chitosan which... Tipo: Info:eu-repo/semantics/article Palavras-chave: Chitosanase; Paenibacillus ehimensis; Chitosanase production; Crude enzymes and TLC. Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000600023 Registros recuperados: 1 Primeira ... 1 ... Última

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