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| Silva,D.G.; Silva,P.R.L.; Fagliari,J.J.; Ávila,F.A; Alessi,A.C.; Oliveira,R.G.. |
| The clinical conditions of healthy calves infected with experimental 10(8)CFU of Salmonella Dublin were evaluated and the viability of the experimental model in disease induction in calves was verified. Twelve 10 to 15-day-old male Holstein calves were examined. They were allocated into two groups, control and experimentally infected with 10(8)CFU of Salmonella Dublin. Animals were submitted to clinical examination after inoculation and at every 12 hours, during seven days after the experimental infection. Samples of rectal swabs were collected for Salmonella Dublin isolation. All animals had severe diarrhea, with mucus and bleeding, 12 to 84 hours after the experimental infection with Salmonella Dublin, accompanied by fever, dehydration and respiratory... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bezerro; Salmonella Dublin; Salmonelose. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352008000100034 |
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| Silva,D.G.; Silva,D.R.; Silva,P.R.L.; Cícero,E.A.S.; Ferraz,A.L.J.; Lemos,M.V.F.; Fagliari,J.J.. |
| The efficiency of the Polymerase Chain Reaction (PCR) combined with selective enrichment broth was compared with the standard microbiological techniques for detection of Salmonella Dublin in fecal samples of 10 to 15-days-old Holstein calves, experimentally infected with 10(8) CFU of Salmonella Dublin. Seventy-six fecal samples were analyzed using PCR associated with selenite cystine (SC) and Muller-Kauffmann tetrathionate (TMK) broths and standard microbiological techniques. Regardless of the selective enrichment broth used, the standard microbiological techniques were significantly better than PCR in detection of positive samples of Salmonella Dublin. However, the simultaneous use of both techniques provided detection of a larger number of positive... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Calves; Feces; Salmonella Dublin; PCR; Culture. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352010000300035 |
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| Santana,A.M.; Fagliari,J.J.; Camargo,C.M.S.; Santana,A.E.; Duarte,J.M.B.; Silva,P.R.L.. |
| The serum protein concentrations of brown brocket deer (Mazama gouazoubira) obtained by agarosis gel and sodium dodecyl sulphate-polyacrylamide (SDS-PAGE) gel were determined from blood samples of ten adult healthy animals (six females and four males), monthly collected in the morning, during 12 months. The animals, maintained in individual stable and protected from noise, received ad libitum a diet of comercial ration and green roughage. Serum protein concentrations in agarosis gel revealed the presence of four protein fractions: albumin, alphaglobulin, betaglobulin, and gammaglobulin. Only serum concentrations of albumin were influenced by season, being values in spring higher than values in summer (4.15 x 3.64g/dl). Serum concentrations of albumin (4.05... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Veado-catingueiro; Proteinograma; Eletroforese; SDS-PAGE. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352008000600039 |
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| Silva,D.G.; Silva,P.R.L.; Fagliari,J.J.. |
| O objetivo do estudo foi avaliar o hemograma e o perfil bioquímico sérico, inclusive hemogasométrico, de bezerros infectados experimentalmente com Salmonella Dublin. Foram utilizados 12 bezerros sadios da raça Holandesa com 10 a 15 dias de idade, distribuídos aleatoriamente em dois grupos experimentais: grupo-controle (n= 6) e grupo infectado com 10(8)UFC de Salmonella Dublin (n=6). Os bezerros foram submetidos ao exame físico diário, e as amostras de sangue foram coletadas minutos antes da inoculação (0h) e 24, 48, 72, 96, 120, 144 e 168h após a inoculação. Além do hemograma e das análises hemogasométricas, foram mensuradas as atividades séricas das enzimas aspartato aminotransferase (AST), fosfatase alcalina (ALP), creatina cinase (CK),... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bezerro; Salmonella Dublin; Diarreia; Hemograma; Eletrólitos. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352010000200002 |
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