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Induction and identification of polyploids in Cattleya intermedia Lindl. (orchidaceae) by in vitro techniques Ciência Rural
Silva,Paulo Artur Konzen Xavier de Mello e; Callegari-Jacques,Sídia; Bodanese-Zanettini,Maria Helena.
The effect of colchicine on the protocorm-like bodies of Cattleya intermedia L. (Orchidaceae) cultured in vitro was studied. Different concentrations of the drug (0.00, 0.05, 0.10 and 0.20%) as well as two times of culture (4 or 8 days) were tested to determine the best treatment for the induction of tetraploid plants. Of the 3 colchicine levels applied, 0.05 and 0.10% of drug treatments appeared to be effective on the production of mixoploids and tetraploids and could be further used in breeding programs. The suitability of stomatal area and stomata density as criteria for the distinction between diploids and tetraploids was also tested. The results suggested that tetraploid plants could be identified with a fair amount of certainty when the screening was...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cattleya; Colchicine; Chromosome number; Orchid improvement; Polyploidy; Stomata analysis.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782000000100017
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