|
|
|
| Registros recuperados: 31 | |
|
| |
|
|
| Silva,Rodrigo Otávio Silveira; Santos,Renata Lara Resende; Pires,Prhiscylla Sadanã; Pereira,Luiz Carlos; Pereira,Silvia Trindade; Duarte,Marina Carvalho; Assis,Ronnie Antunes de; Lobato,Francisco Carlos Faria. |
| The objective of this study was to detect C. difficileA/B toxins and to isolate strains of C. perfringensand C. difficile from diarrheic and non-diarrheic dogs in Brazil. Stool samples were collected from 57 dogs, 35 of which were apparently healthy, and 22 of which were diarrheic. C. difficileA/B toxins were detected by ELISA, and C. perfringensand C. difficilewere identified by multiplex PCR. C. difficileA/B toxins were detected in 21 samples (36.8%). Of these, 16 (76.2%) were from diarrheic dogs, and five (23.8%) were from non-diarrheic dogs. Twelve C. difficile strains (21.1%) were isolated, of which ten were A+B+and two were A-B-. All non-toxigenic strains were isolated from non-diarrheic animals. The binary toxin gene cdtBwas found in one strain,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Nosocomial diarrhea; Small animals; Colitis; Enteritis; Canine. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000100019 |
| |
|
|
| Alves,Guilherme Guerra; Machado-de-Ávila,Ricardo Andrez; Chávez-Olórtegui,Carlos Delfin; Silva,Rodrigo Otávio Silveira; Lobato,Francisco Carlos Faria. |
| Abstract The epsilon toxin, produced by Clostridium perfringens, is responsible for enterotoxemia in ruminants and is a potential bioterrorism agent. In the present study, 15 regions of the toxin were recognized by antibodies present in the serum, with different immunodominance scales, and may be antigen determinants that can be used to formulate subunit vaccines. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Epsilon; Toxin; Enterotoxemia; Epitope; Mapping. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000300570 |
| |
|
|
| Casagrande,Renata Assis; Pires,Prhiscylla Sadanã; Silva,Rodrigo Otávio Silveira; Sonne,Luciana; Borges,João Batista Souza; Neves,Monique Silva; Rolim,Veronica Machado; Souza,Suyene Oltramari de; Driemeier,David; Lobato,Francisco Carlos Faria. |
| The aim of this study was to report a case of clostridial myocarditis in a bovine in Brazil with emphasis on the pathological findings, isolation and molecular identification associated with the in situ localization of C. chauvoei. The animal, a male Brangus bull with nine months of age, was found dead without prior clinical signs. Multifocal and coalescent areas of necrosis were observed in the myocardium. Rod cells in the cardiac muscle fibers were positive immunostaining for C. chauvoei, while this bacterium was also isolated and identified by polymerase chain reaction (PCR). |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Myonecrosis; Sudden death; Clostridium chauvoei; Myocarditis.. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782015000801472 |
| |
|
|
| Pires,Prhiscylla Sadanã; Uribe,Jose Azael Zambrano; Carvalho,Antônio Último de; Silva,Rodrigo Otávio Silveira; Salvarani,Felipe Masiero; Meneses,Rodrigo Melo; Leite,Luiza Bossi; Alves,Guilherme Guerra; Gonçalves,Luciana Aramuni; Lobato,Francisco Carlos Faria; Facury Filho,Elias Jorge. |
| Clostridium perfringenstype A has been incriminated as the etiologic agent in jejunal hemorrhage syndrome (JHS), which is a disease that affects dairy cattle. Although this microorganism is considered an important enteropathogen the pathogenesis of JHS is still not clear, and there have been no reports of its occurrence in Brazil so far. The aim of this study was to describe the occurrence of JHS by infection with a C. perfringenstype A strain carrying the beta-2 toxin gene in a zebu cow in Brazil, for the first time. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Clostridium perfringens; Beta-2 toxin gene; Enteropathogen; Enteric blood clot.. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782015000801476 |
| |
|
|
| Silva,Rodrigo Otávio Silveira; Moreira,Felipe Moraghi; Rezende,Jackeline Viana; Pires,Prhiscylla Sadanã; Maranhão,Renata de Pino Albuquerque; Palhares,Maristela Silveira; Lobato,Francisco Carlos Faria. |
| Despite of the substantial role of Clostridium difficile in causing diarrhea and colitis in foals, there have been no confirmed diagnoses of disease caused by this bacteria in Brazil. In this paper, we describe confirmed cases of colitis caused by C. difficile in two foals in Brazil. Two five-month-old foals with a five-day history of diarrhea after antibiotic treatment for a respiratory disease were treated at the Veterinary Hospital of the Universidade Federal de Minas Gerais. C. difficile A/B toxins were detected, and toxigenic strains of C. difficile were isolated from the foals' feces. The treatment was based on fluid therapy and antibiotics (metronidazole and ceftiofur), and the animals experienced a gradual recovery. The association between the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Colitis; Equine; Nosocomial diarrhea. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782012000300018 |
| |
|
|
| Silva,Rodrigo Otávio Silveira; Oliveira Junior,Carlos Augusto; Diniz,Amanda Nádia; Alves,Guilherme Guerra; Guedes,Roberto Maurício Carvalho; Vilela,Eduardo Garcia; Lobato,Francisco Carlos Faria. |
| The objective of this study was to evaluate antimicrobial susceptibility in Clostridium difficile strains isolated from animals and humans in Brazil. The 54 C. difficile strains used were isolated from stool samples from piglets (n=16), dogs (n=13), humans (n=13), foals (n=8) calves (n=2), an ocelot (n=1) and a maned wolf (n=1). Antimicrobial susceptibility was determined using the serial plate agar dilution method for penicillin, florfenicol, oxytetracycline, erythromycin, vancomycin, metronidazole and tylosin. The C. difficile strains assessed were susceptible to metronidazole and vancomycin. Florfenicol resistance was rarely observed; 52 (96.4%) strains were sensitive to this antimicrobial. Five (9.3%), five (9.3%), 14 (25.9%) and 20 (37.0%) strains... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Nosocomial diarrhea; Pseudomembranous colitis; Resistance. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782014000500013 |
| |
|
|
| Ramos,Carolina Pantuzza; Vespasiano,Letícia Camêlo; Melo,Isabela Oliveira; Xavier,Rafael Gariglio Clark; Leal,Carlos Augusto Gomes; Facury Filho,Elias Jorge; Carvalho,Antônio Ultimo de; Lobato,Francisco Carlos Faria; Silva,Rodrigo Otávio Silveira. |
| ABSTRACT: The present study aimed to describe and characterize a nosocomial outbreak caused by a multidrug resistant Salmonella Typhimurium in hospitalized calves at a veterinary medical teaching hospital from Brazil. Sixty-three (96.9%) calves showed lethargy, hyperthermia and profuse diarrhea and despite treatment, 26 (41.2%) animals died. Five animals were necropsied and stool samples of six calves were collected. The isolated strains were subjected to antimicrobial susceptibility test by disc-difusion method and were fingerprinted by ERIC-PCR. Macroscopic lesions suggestive of salmonellosis, such as fibrinonecrotic enteritis and hepatosplenomegaly were observed. Salmonellosis was confirmed by isolation of S. Typhimurium from stool samples and organs... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Salmonellosis; Bovine; Nosocomial; Zoonosis; Multiresistant. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019000200452 |
| |
|
|
| Silva,Rodrigo Otávio Silveira; Salvarani,Felipe Masiero; Cruz Júnior,Eduardo Coulaud da Costa; Pires,Prhiscylla Sadanã; Santos,Renata Lara Resende; Assis,Ronnie Antunes de; Guedes,Roberto Maurício de Carvalho; Lobato,Francisco Carlos Faria. |
| Clostridium difficile has emerged as a major cause of neonatal colitis in piglets, displacing classic bacterial pathogens. However, there is no information regarding the distribution of this microorganism in pig farms in Brazil. In the present study, the presence of toxins A/B and of C. difficile strains in stool samples from 60 diarrheic or non-diarrheic newborn piglets (one to seven days old), from 15 different farms, was studied. The presence of toxins A/B was detected by ELISA and PCR was used to identify toxin A, toxin B and binary toxin gene in each isolated strain. C. difficile A/B toxins were detected in ten samples (16.7%). Of these, seven were from diarrheic and three were from non-diarrheic piglets. C. difficile was recovered from 12 out of 60... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Neonatal diarrhea; Clostridium difficile; Enteritis; Colitis. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782011000800021 |
| |
|
| |
|
|
| Lobato,Francisco Carlos Faria; Salvarani,Felipe Masiero; Silva,Rodrigo Otávio Silveira; Assis,Ronnie Antunes de; Lago,Luis Alberto do; Carvalho Filho,Maurício Baltazar de; Martins,Nelson Rodrigo da Silva. |
| O botulismo é uma intoxicação causada pela ingestão das toxinas produzidas pelo Clostridium botulinum, que acomete mamíferos e aves e é caracterizado por um quadro de paralisia flácida. Neste trabalho, é descrito um surto de botulismo em perus, ocorrido no município de Santa Luzia, região metropolitana de Belo Horizonte, Minas Gerais. Os animais apresentavam incoordenação motora, paralisia flácida das patas, asas e pescoço. Em um intervalo de 24 horas, todos os 29 animais do plantel vieram a óbito. Na necropsia, observou-se a presença de larvas de mosca no inglúvio. Nos soros coletados, foi identificada a toxina botulínica tipo C pelo teste de soroneutralização em camundongos. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Toxina botulínica tipo C; Clostridium botulinum; Perus; Aves. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782009000100047 |
| |
|
|
| Silva,Rodrigo Otávio Silveira; Gabardo,Michelle de Paula; Oliveira,Juliana Saes Vilaça de; Lobato,Francisco Carlos Faria; Guedes,Roberto Maurício Carvalho. |
| Lawsonia intracellularisis an obligate intracellular bacterium that is responsible for proliferative enteropathy, an enteric disease endemic in swine and common in foals. However, few studies have investigated this disease in dogs, and there are no reports of dogs infected with L. intracellularisin Latin America. The aim of this study was to evaluate the fecal shedding of L. intracellularisin diarrheic and non-diarrheic dogs in Minas Gerais, Brazil. A total of 58 dogs, 18 apparently healthy and 40 diarrheic, were examined in this study. DNA extracted from feces was analyzed using a nested PCR reaction to detect L. intracellularis.Three out of 40 (7.5%) diarrheic samples, all from 3-month-old puppies, were positive for L. intracellularis. These results... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Lawsonia intracellularis; Canine; Proliferative enteropathy; Diarrhea. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782015000901619 |
| |
|
|
| Pires,Prhiscylla Sadanã; Ecco,Roselene; Silva,Rodrigo Otávio Silveira; Araújo,Marina Rios de; Salvarani,Felipe Masiero; Heneine,Luíz Guilherme Dias; Oliveira Júnior,Carlos Augusto de; Lobato,Francisco Carlos Faria. |
| ABSTRACT: A standardized immunochemistry method for the diagnosis of clostridial myonecrosis was applied to 38 formalized tissue samples from ruminants with clinical and post mortem history suggestive of blackleg or gas gangrene. The diagnosis of clostridial myonecrosis was confirmed in 37 out of 38 (97.4%) samples tested. Clostridium chauvoei and Clostridium perfringens type A were the most common agents found alone, being detected in ten (26.3%) and six (15.8%) samples, respectively. The other cases showed an association of two or three clostridia, with C. perfringens type A detected in 11 (29%) cases. Based on the findings of the present study, polyvalent vaccines against clostridial infections of animals incorporating C. perfringens would be more... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Gas gangrene; Blackleg; Malignant edema; Cattle; Sheep; Immunohistochemistry. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017000100452 |
| |
|
|
| Freitas,Theonys Diógenes; Heneine,Luis Guilherme Dias; Salvarani,Felipe Masiero; Silva,Rodrigo Otávio Silveira; Lima,Catarina Guimarães Rocha Dourado; Pires,Prhiscylla Sadanã; Assis,Ronnie Antunes de; Lobato,Francisco Carlos Faria. |
| Clostridium perfringens tipo D é o agente etiológico da enterotoxemia em ruminantes, causada pela toxina épsilon e caracterizada por edema cardíaco, pulmonar, renal e cerebral. Anticorpos monoclonais contra toxina épsilon de C. perfringens tipo D foram produzidos a partir da fusão da linhagen de mieloma P3-X63-Ag8 653 com células do baço de camundongos Balb/c imunizados com o toxóide épsilon. Seis linhagens de híbridos secretores de anticorpos monoclonais das classes e IgM e IgG foram estabelecidas. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Clostridium perfringens; Toxina épsilon; Anticorpos monoclonais. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782009000100046 |
| |
|
|
| Pinheiro,Carolina de Gouveia Mendes da Escóssia; Abrantes,Maria Rociene; Silva,Rodrigo Otávio Silveira; Oliveira Junior,Carlos Augusto; Lobato,Francisco Carlos Faria; Silva,Jean Berg Alves da. |
| ABSTRACT: The aim of this study was to evaluate the presence of microorganisms in honey produced by the stingless bee (SB) jandaíra (Melipona subnitida) from the semiarid region of Brazil. Thirty-five samples were analyzed and all of them were positive for mesophilic bacteria, coliforms at 45°C, fungi, and yeast. Staphylococcus spp. were identified in 85.7%, while Bacillus spp. were detected in 34.3% of honey samples. DNAs of Clostridium perfringens and C. botulinum were detected in 40% and 2.8% of the samples, respectively. Salmonella spp. and C. difficile were not detected. The present research revealed a great diversity of microorganisms in honey produced by jandaíra. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Clostridium botulinum; Clostridium perfringens; Stingless bee. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000900450 |
| |
|
|
| Ramos,Carolina Pantuzza; Xavier,Rafael Gariglio Clark; Leal,Carlos Augusto Gomes; Facury Filho,Elias Jorge; Carvalho,Antonio Ultimo de; Viegas,Flávia Mello; Pires,Isadora Honorato; Lopes,Emily Oliveira; Lobato,Francisco Carlos Faria; Silva,Rodrigo Otávio Silveira. |
| ABSTRACT: The present study aimed to describe and characterize, for the first time, two outbreaks of salmonellosis caused by Salmonella Ndolo in foals and calves in Brazil and compare the isolated strains with S. Ndolo previously identified in asymptomatic reptiles. The affected calves and foals presented fever, lethargy, and profuse diarrhea. Isolated strains were subjected to antimicrobial susceptibility testing, characterized according to virulence genes, and fingerprinted by ERIC-PCR. Salmonella Ndolo was identified in fecal samples from two foals and four calves. One isolate from a calf was resistant to amoxicillin/clavulanic acid, trimethoprim/sulfamethoxazole, and florfenicol. Strains from two other calves were resistant to oxytetracycline. All... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Salmonellosis; Zoonoses; Antimicrobial resistance; ERIC-PCR; Salmonella Ndolo.. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018001200452 |
| |
|
| |
|
| |
|
|
| Silva,Rodrigo Otávio Silveira; Oliveira Junior,Carlos Augusto; Guedes,Roberto Maurício Carvalho; Lobato,Francisco Carlos Faria. |
| Clostridium perfringens is a gram-positive, anaerobic, fermentative, spore-forming bacillus that may be found in the environment but is more commonly found as part of the microbiota of humans and animals. The bacterium is considered a common enteric pathogen; however, the pathogenesis and predisposing factors of the disease can differ among species. Therefore, specific studies are necessary for understanding the role of this pathogen, how to diagnose it and which control measures are applicable. The aim of this paper is to review the current knowledge regarding C. perfringens infections in pigs, horses and broiler chickens |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Diarrhea; Enteritis; Equine; Swine; Poultry. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782015000601027 |
| |
|
|
| Lobato,Francisco Carlos Faria; Salvarani,Felipe Masiero; Silva,Rodrigo Otávio Silveira; Souza,Andréa Márcia de; Lima,Catarina Guimarães Rocha Dourado; Pires,Prhiscylla Sadanã; Assis,Ronnie Antunes de; Azevedo,Edisio Oliveira de. |
| Botulismo é uma intoxicação causada pela ingestão das toxinas produzidas pelo Clostridium botulinum, que acomete mamíferos e aves. Neste trabalho é descrito um surto de botulismo em ruminantes, ocorrido em duas propriedades localizadas no município de Patos, no Estado da Paraíba, Brasil. Em uma das propriedades, de um total de 88 bovinos, 85 (96,6%) vieram a óbito. Na segunda, morreram 145 ovinos (96,7%), 233 caprinos (57,8%) e 30 bovinos (96,8%). Os animais acometidos apresentavam paralisia progressiva, dificuldade de locomoção, sialorréia e dispnéia. A morte ocorreu entre 24 e 48 horas após o início dos sinais, por parada cardio-respiratória. Nenhuma alteração significativa foi observada no exame post-mortem. O diagnóstico de botulismo foi confirmado... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Botulismo; Clostridium botulinum; Cama-de-frango; Ruminantes. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782008000400046 |
| |
| Registros recuperados: 31 | |
|
|
|
