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| Sanson,Ananda Lima; Silva,Suéllen Cristina Rennó; Martins,Matheus Coutinho Gonçalves; Giusti-Paiva,Alexandre; Maia,Patrícia Penido; Martins,Isarita. |
| A liquid-liquid extraction (LLE) combined with high-performance liquid chromatography-diode array detection method for simultaneous analysis of four chemically and structurally different antineoplastic drugs (cyclophosphamide, doxorubicin, 5-fluorouracil and ifosfamide) was developed. The assay was performed by isocratic elution, with a C18 column (5 µm, 250 x 4.6 mm) and mobile phase constituted by water pH 4.0- acetonitrile-methanol (68:19:13, v/v/v), which allowed satisfactory separation of the compounds of interest. LLE, with ethyl acetate, was used for sample clean-up with recoveries ranging from 60 to 98%. The linear ranges were from 0.5 to 100 µg mL-1, for doxorubicin and 1 to 100 µg mL-1, for the other compounds. The relative standard deviations... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Antineoplastic drugs/simultaneous determination in plasma; Liquid-liquid extraction; High-performance liquid chromatography/diode array detector. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502011000200017 |
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