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Microplanktonic Community Structure in a Coastal System Relative to a Phaeocystis Bloom Inferred from Morphological and Tag Pyrosequencing Methods ArchiMer
Monchy, Sebastien; Grattepanche, Jean-david; Breton, Elsa; Meloni, Dionigia; Sanciu, Giovanna; Chabe, Magali; Delhaes, Laurence; Viscogliosi, Eric; Sime-ngando, Telesphore; Christaki, Urania.
Background: Massive phytoplankton blooms, like the recurrent Phaeocystis proliferation observed every year in the Eastern English Channel (EEC), have a significant influence on the overall planktonic community structure and their food web dynamics. As well as being an important area for local fisheries, the EEC is an ideal ecosystem for work on microbial diversity. This is because, although its environmental context is relatively complex, it is reasonably well understood due to several years of monitoring and morphological observations of its planktonic organisms. The objective of our study was to better understand the under-explored microbial eukaryotic diversity relative to the Phaeocystis bloom. Methodology and Principal Findings: The community...
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Ano: 2012 URL: https://archimer.ifremer.fr/doc/00373/48460/48724.pdf
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Seasonal variations of marine protist community structure based on taxon-specific traits using the eastern English Channel as a model coastal system ArchiMer
Genitsaris, Savvas; Monchy, Sebastien; Viscogliosi, Eric; Sime-ngando, Telesphore; Ferreira, Stephanie; Christaki, Urania.
Previous microscopy-based studies in the eastern English Channel have revealed it to be a productive meso-eutrophic coastal ecosystem, characterized by strong repeating patterns in microplankton succession. The present study examines the seasonal structure of the entire protistan community from March 2011 to July 2013, using tag pyrosequencing of the V2-V3 hypervariable region of the 18S rRNA gene. A total of 1242 OTUs and 28 high-level taxonomic groups, which included previously undetected taxa in the area, were identified. The detected OTUs were considered according to taxon-specific traits, which included their trophic role, abundance and specialization level. Taxa differentiation based on specialization level rather than abundance was more informative...
Tipo: Text Palavras-chave: Unicellular eukaryotes; Tag pyrosequencing; 18S rRNA gene; Succession; Generalists; Specialists.
Ano: 2015 URL: https://archimer.ifremer.fr/doc/00373/48454/48738.pdf
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Microbial food web structural and functional responses to oyster and fish as top predators ArchiMer
Mostajir, Behzad; Roques, Cecile; Bouvier, Corinne; Bouvier, Thierry; Fouilland, Eric; Got, Patrice; Le Floc'H, Emilie; Nouguier, Jean; Mas, Sebastien; Sempere, Richard; Sime-ngando, Telesphore; Troussellier, Marc; Vidussi, Francesca.
The impact of fish and oysters on components of the pelagic microbial food web (MFW) was studied in a 10 d mesocosm experiment using Mediterranean coastal waters. Two mesocosms contained natural water only ('Controls'), 2 contained natural water with Crassostrea gigas ('Oyster'), and 2 contained natural water with Atherina spp. ('Fish'). Abundances and biomasses of microorganisms (viruses, bacteria, phytoplankton, heterotrophic flagellates, and ciliates) were measured to estimate their contribution to the total microbial carbon biomass. Two MFW indices, the microbial autotroph: heterotroph C biomass ratio (A:H) structural index and the gross primary production: respiration ratio (GPP:R) functional index, were defined. In the Fish mesocosms, selective...
Tipo: Text Palavras-chave: Microbial food web; Virioplankton; Bacterioplankton; Phytoplankton; Protozooplankton; Crassostrea; Atherina; Autotrophy; Heterotrophy.
Ano: 2015 URL: https://archimer.ifremer.fr/doc/00284/39489/73675.pdf
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