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	Provedor de dados BJM (3) Autor Simonetti,Amauri Braga (3) Roehe,Paulo Michel (2) Campos,Karen (1) David,Cíntia de (1) Dezen,Diogenes (1) Englert,Gelsa Edith (1) Kwitko,Sérgio (1) Locatelli,Claudete I. (1) Mergener,Michelle (1) Oliveira,Anna Paula de (1) Spilki,Fernando Rosado (1) Vaucher,Rodrigo de Almeida (1) Mais... Palavra-chave Antibiotic (1) BPIV-3 (1) Bacteria (1) Bovine parainfluenza virus type 3 (1) Cataract (1) Cell culture (1) Conjunctiva (1) Contamination (1) Fetal bovine serum (FBS) (1) Intraocular lens (1) Nanobacteria (1) Phylogenetic analysis (1) Mais... Tipo do documento Info:eu-repo/semantics/article (3) Ano 2011 (1) 2007 (1) 2003 (1) País Brazil (3) Idioma Inglês (3)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 3 Primeira ... 1 ... Última Phylogenetic characterization of bovine parainfluenza 3 from contaminated cell cultures and field isolates from Brazil BJM Vaucher,Rodrigo de Almeida; Dezen,Diogenes; Simonetti,Amauri Braga; Spilki,Fernando Rosado; Roehe,Paulo Michel. Genomic fragments of the HN and L genes from Brazilian bovine parainfluenza 3 virus (bPIV-3) isolated as contaminants from cell cultures and clinical specimens were amplified by reverse transcription-polymerase chain reaction (RT-PCR), sequenced using specific degenerate primers and analyzed by phylogenetic comparison with reference strains of bPI3V. The Brazilian isolates revealed a high degree of genomic when compared to SF4/32 prototype strain, within the recently proposed genotype A of bPIV-3. Tipo: Info:eu-repo/semantics/article Palavras-chave: Bovine parainfluenza virus type 3; BPIV-3; Phylogenetic analysis. Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000400029 Conjunctival endogenous microbiota in patients submitted to cataract surgery BJM Locatelli,Claudete I.; Kwitko,Sérgio; Simonetti,Amauri Braga. Bacterial isolation, identification and antimicrobial susceptibility tests were carried out in ocular material collected with swab and polimethylmethacrylate (PMMA) or silicone intraocular lenses (IOL) from forty six patients submitted to cataract surgery. Seventy six isolates and seven different microorganisms were identified. Coagulase-negative staphylococci (CNS) were the predominant microorganisms isolated from swabs (71.4% of cases), PMMA lenses (81.3%) and silicon lenses (77.8%). Coagulase-negative staphylococci isolates revealed high resistance to penicillin G followed by tetracycline, chloramphenicol and aminoglicosides. However, these isolates displayed great susceptibility to vancomycin, cephalothin and ofloxacin. Except for penicillin G,... Tipo: Info:eu-repo/semantics/article Palavras-chave: Bacteria; Conjunctiva; Cataract; Intraocular lens; Antibiotic. Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000300004 Nanobacteria-like particles: a threat to cell cultures BJM Simonetti,Amauri Braga; Englert,Gelsa Edith; Campos,Karen; Mergener,Michelle; David,Cíntia de; Oliveira,Anna Paula de; Roehe,Paulo Michel. The main goal of this study is to alert researchers who work with cell cultures for the risk of contamination by structures called nanobacteria (NB). NB are tiny structures with size varying from 80 to 500 nm, commonly occurring in clusters and producing a biofilm which contains carbonate or hydroxyl apatite. The most likely source of cell culture contamination by such organisms is serum used as supplement in culture media. The presence of NB leads to a progressive culture deterioration with accumulation of granules (probably phagocytized NB) in cytoplasmic vacuoles, an increasing number of dead cells in the supernatant and degeneration of cells that remained attached to the bottom of the vessel. NB can also be found in culture supernatants where they are... Tipo: Info:eu-repo/semantics/article Palavras-chave: Nanobacteria; Cell culture; Fetal bovine serum (FBS); Contamination. Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000100032 Registros recuperados: 3 Primeira ... 1 ... Última

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