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	Provedor de dados 58 (2) Autor Singh,Satya P. (2) Dodia,Mital S. (1) Joshi,Rupal H. (1) Patel,Rajesh K. (1) Thumar,Jignasha T. (1) Palavra-chave Alkaline protease (2) Alkaliphiles (1) Enzymatic potential (1) Extremophiles (1) Halophiles (1) Protease optimization (1) Protease stability (1) Protein folding (1) Salt-tolerant actinomycetes (1) Streptomyces clavuligerus (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2007 (1) 2006 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Characterization and stability of extracellular alkaline proteases from halophilic and alkaliphilic bacteria isolated from saline habitat of coastal Gujarat, India 58 Dodia,Mital S.; Joshi,Rupal H.; Patel,Rajesh K.; Singh,Satya P.. The present study deals with the isolation and characterization of the moderately halophilic-alkaliphilic bacteria from a saline habitat in western India. Eight different bacterial strains were isolated using enrichment techniques at 20% (w/v) NaCl and pH 10. The isolates exhibited diversity towards gram's reaction, colony and cell morphology. They were able to grow and produce alkaline protease over a broad range of NaCl, 5-20% (w/v) and pH, 8-10. None of the isolates could grow at pH 7, and one could not grow even at pH 8. Crude and partially purified proteases from strain S5 were subjected to characterization with reference to pH, salt stability and protein folding. Optimum protease activity and stability was recorded at 10% salt and pH 9-9.5.... Tipo: Info:eu-repo/semantics/article Palavras-chave: Halophiles; Alkaliphiles; Alkaline protease; Protease stability; Protein folding; Extremophiles. Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000300015 Secretion of an alkaline protease from a salt- tolerant and alkaliphilic, Streptomyces clavuligerus strain Mit-1 58 Thumar,Jignasha T.; Singh,Satya P.. An alkaliphilic and salt- tolerant actinomycete, Streptomyces clavuligerus strain Mit-1, was isolated from Mithapur, the western coast of India. The organism was Gram-positive, having filamentous, long thread like structure. The sporulation started after two days of growth and the optimum level of alkaline protease (130 U/ml) was produced during the early stationary phase. The strain could grow and produce protease with 0-10% NaCl (w/v), the optimum being 5% NaCl (w/v). Growth and protease production was optimum at pH 9 with substantial decline at neutral pH. Sucrose and gelatin were the best carbon and nitrogen sources respectively, whereas gelatin broth was the preferred medium for protease production. Mit-1 produced substantial protease with various... Tipo: Info:eu-repo/semantics/article Palavras-chave: Streptomyces clavuligerus; Salt-tolerant actinomycetes; Alkaline protease; Enzymatic potential; Protease optimization. Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000400033 Registros recuperados: 2 Primeira ... 1 ... Última

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