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| Sgarioni,Sônia Aparecida; Hirata,Rosario Dominguez Crespo; Hiroyuki Hirata,Mario; Leite,Clarice Queico Fujimura; Prince,KarinaAndrade de; Leite,Sergio Roberto de Andrade; Vedovello Filho,Dirceu; Siqueira,Vera Lucia Dias; Caleffi-Ferracioli,Katiany Rizzieri; Cardoso,Rosilene Fressatti. |
| Milk is widely consumed in Brazil and can be the vehicle of agent transmission. In this study, was evaluated the occurrence of Mycobacterium bovis and non-tuberculous mycobacteria (NTM) in raw and pasteurized milk consumed in the northwestern region of Paraná, Brazil. Fifty-two milk samples (20 pasteurized and 32 raw) from dairy farms near the municipality of Maringa, Parana State, Brazil were collected. Milk samples were decontaminated using 5% oxalic acid method and cultured on Lowenstein-Jensen and Stonebrink media at 35 °C and 30 °C, with and without 5-10% CO2. Mycobacteria isolates were identified by morphological features, PCR-Restriction Fragment Length Polymorphism Analysis (PCR-PRA) and Mycolic acids analysis. Thirteen (25%) raw and 2 (4%)... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Non-tuberculous mycobacteria; Milk; Mycobacterium; PCR-PRA; Mycolic acids analysis. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000200046 |
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| Leite,Clariana Akemi Kariya; Oizumi,Karina Yoshimi; Caleffi-Ferracioli,Katiany Rizzieri; Scodro,Regiane Bertin de Lima; Pádua,Rubia Andreia Falleiros de; Cardoso,Rosilene Fressatti; Pires,Claudia Terencio Agostinho; Siqueira,Vera Lucia Dias. |
| ABSTRACT The present study evaluated the antimicrobial susceptibility profile, β-lactamase production, and genetic diversity of Enterobacteriaceae, Pseudomonas aeruginosa, and Acinetobacter spp. using phenotypic identification, antimicrobial susceptibility testing, and β-lactamase phenotypic detection. Isolates were obtained from patients in an intensive care unit in a hospital in southern Brazil. Bacterial genomic DNA was extracted, followed by the genotypic detection of carbapenemases and enterobacterial repetitive intergenic consensus-polymerase chain reaction (ERIC-PCR). Fifty-six isolates (26 Klebsiella pneumoniae, five Escherichia coli, three Enterobacter aerogenes, nine P. aeruginosa, and 13 Acinetobacter spp.) were evaluated. The phenotypic... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Enterobacteriaceae; Pseudomonas aeruginosa; Acinetobacter spp; Antimicrobial resistance; Beta-lactamases; Bacterial typing; Intensive Care Unit/study/Brazil; Drug-resistant bacteria.. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000200609 |
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| Meneguello,Jean Eduardo; Silva,João Vitor de Oliveira; Lima,Letícia Silva; Siqueira,Vera Lucia Dias; Scodro,Regiane Bertin de Lima; Caleffi-Ferracioli,Katiany Rizzieri; Cardoso,Rosilene Fressatti. |
| ABSTRACT Microscopy and bacterial culture are the main tools in the diagnosis of tuberculosis. Since the slow growth of Mycobacterium tuberculosis impairs rapid diagnosis strategies, especially in countries where the latter are the only available resources, the ongoing development of new and inexpensive tools based on mycobacterial metabolism optimizing growth detection with preliminary identification is greatly welcome. When compared to the other species from the M. tuberculosis complex, M. tuberculosis is a strong nitrate reducer. Current assay compares the nitrate reductase activity of M. tuberculosis from pulmonary specimens cultivated in nitrate-supplemented media. Fifty-five sputum samples were decontaminated and inoculated in conventional... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Tuberculosis; Mycobacteria/diagnosis/identification; Nitrate reductase. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000100602 |
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| Amaral,Renata Claro Ribeiro do; Caleffi-Ferracioli,Katiany Rizzieri; Demitto,Fernanda de Oliveira; Almeida,Aryadne Larissa de; Siqueira,Vera Lucia Dias; Scodro,Regiane Bertin de Lima; Leite,Clarice Queico Fujimura; Pavan,Fernando Rogério; Cardoso,Rosilene Fressatti. |
| The membrane-based efflux pump systems are recognized to have an important role in pathogenicity and drug resistance in Mycobacterium tuberculosis by the extrusion of toxic substrates and drugs from the inner bacillus. This study aimed to investigate the in vitro interaction of Verapamil (VP), an efflux pump inhibitor, with the classical first-line anti-tuberculosis drug isoniazid (INH) in resistant and susceptible M. tuberculosis clinical isolates. Seven multidrug-resistant (MDR), three INH monoresistant and four susceptible M. tuberculosis clinical isolates were tested for the INH and VP combination by modified Resazurin Microtiter Assay Plate (REMA). Fractional Inhibitory Concentration (FIC) and Modulation Factor (MF) were determined. The INH plus VP... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Tuberculosis; Multidrug-resistance; Efflux pumps; Efflux pumps inhibitors; Isoniazid. |
| Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100588 |
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| Caleffi-Ferracioli,Katiany Rizzieri; Baldin,Vanessa Pietrowski; Noguti,Érika Noda; Siqueira,Vera Lucia Dias; Scodro,Regiane Bertin Lima; Pavan,Fernando Rogério; Hirata,Mario Hiroyuki; Cardoso,Rosilene Fressatti. |
| Tuberculosis (TB) is an infectious disease in which the molecular typing methods allow to have important information about the dynamics of transmission and to assist properly in disease control. Although the ERIC-PCR (Enterobacterial repetitive intergenic consensus-PCR) assay is fast and easy to perform, scarce studies have reported its use in epidemiological studies in TB outbreaks. In this study, we aimed to genotype Mycobacterium tuberculosis and M. bovis isolates by ERIC-PCR and compare its discriminatory power with two other classically used methods: 12 loci-MIRU (Mycobacterial Interspersed Repetitive Units) and Spoligotyping. The M. tuberculosis isolates studied were from northwestern and southwestern and M. bovis from northwestern Parana, Brazil.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Tuberculosis; Molecular characterization; ERIC-PCR; Epidemiology. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000300616 |
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