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Isolation and multiplex genotyping of polymorphic microsatellite DNA markers in the snakehead murrel, Channa striata Genet. Mol. Biol.
Jamsari,Amirul Firdaus Jamaluddin; Min-Pau,Tan; Siti-Azizah,Mohd Nor.
Seven polymorphic microsatellite loci were isolated and characterized for the snakehead murrel, Channa striata (Channidae), a valuable tropical freshwater fish species. Among 25 specimens collected from Kedah state in Malaysia, the number of alleles per locus ranged from 2 to 7. Observed and expected heterozygosities ranged from 0.120 to 0.880 and 0.117 to 0.698, respectively. A single locus (CS1-C07) was significantly deviated from Hardy-Weinberg equilibrium after Bonferroni correction. These novel markers would be useful for population genetic studies of the C. striata.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Channa striata; Population genetics; Microsatellite; Multiplex PCR.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000200029
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Genetic structure of the snakehead murrel, Channa striata (channidae) based on the cytochrome c oxidase subunit I gene: influence of historical and geomorphological factors Genet. Mol. Biol.
Jamaluddin,Jamsari Amirul Firdaus; Pau,Tan Min; Siti-Azizah,Mohd Nor.
Nucleotide sequences of a partial cytochrome c oxidase subunit I gene were used to assess the manner in which historical processes and geomorphological effects may have influenced genetic structuring and phylogeographic patterns in Channa striata. Assaying was based on individuals from twelve populations in four river systems, which were separated into two regions, the eastern and western, of the biodiversely rich state of Perak in central Peninsular Malaysia. In 238 specimens, a total of 368-bp sequences with ten polymorphic sites and eleven unique haplotypes were detected. Data on all the twelve populations revealed incomplete divergence due to past historical coalescence and the short period of separation. Nevertheless, SAMOVA and F ST revealed...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Channa striata; MtDNA COI; Phylogeography; Population structure.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000100026
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Evidence of geographical structuring in the Malaysian Snakehead, Channa striata based on partial segment of the CO1 gene Genet. Mol. Biol.
Siti-Balkhis,Abu Bakar; Jamsari,Amirul Firdaus Jamaluddin; Hwai,Tan Shau; Yasin,Zulfigar; Siti-Azizah,Mohd Nor.
Channa striata, locally known as "haruan", is economically important in fisheries and aquaculture industries in several Asian countries. DNA sequencing, based on a partial segment of the Cytochrome oxidase c subunit 1 (CO1) gene, was used to determine genetic variation in C. striata samples from four different populations on the west coast of Peninsular Malaysia. The highest nucleotide and haplotype diversities were observed in the Linggi population (π = 0.0067, h = 0.835), and the lowest in the Timah Tasoh population (π = 0.0008, h = 0.286). Apart from Kajang-Linggi, which was insignificant, F ST values were significant (p < 0.05) in all pairwise-population comparisons. Consequently, it is inferred that genetic structuring C. striata populations in...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Channa striata; Snakehead; MtDNA; CO1; Isolation by distance.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000300024
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