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Use of endophytic diazotrophic bacteria as a vector to express the cry3A gene from Bacillus thuringiensis BJM
Salles,Joana Falcão; Gitahy,Patrícia de Medeiros; Skøt,Leif; Baldani,José Ivo.
The goal of this study was to evaluate the potential of endophytic diazotrophic bacteria as a vector to express a cry gene from Bacillus thuringiensis, envisaging the control of pests that attack sugarcane plants. The endophytic nitrogen-fixing bacteria Gluconacetobacter diazotrophicus strain BR11281 and Herbaspirillum seropedicae strain BR11335 were used as models. The cry3A gene was transferred by conjugation using a suicide plasmid and the recombinant strains were selected by their ability to fix nitrogen in semi-solid N-free medium. The presence of the cry gene was detected by Southern-blot using an internal fragment of 1.0 kb as a probe. The production of delta-endotoxin by the recombinant H. seropedicae strain was detected by dot blot while for G....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Gluconacetobacter diazotrophicus; Herbaspirillum seropedicae; Endophytic bacteria; Diazotrophic; Cry gene.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000300001
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