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Application of Scharer's quantitative method for the determination of residual alkaline phosphatase activity in standard Minas Arq. Bras. Med. Vet. Zootec.
Soares,C.F.; Fonseca,L.M.; Leite,M.O.; Oliveira,M.C.P.P..
Milk pasteurization is a critical issue in the dairy industry, and failures in this process can affect final product safety. Scharer's enzymatic method is still traditionally used to verify pasteurization efficiency compliance, and it is based on screening for residual alkaline phosphatase in milk. Although several methods are used to quantify enzymatic activity to assess milk pasteurization efficiency, there is a small amount of published data regarding the use of these methods to quantify alkaline phosphatase in cheese. In this study, the Scharer's modified method was used to determine the levels of residual alkaline phosphatase in standard minas cheese, before and after 20 days of ripening. The cheeses were made using raw or pasteurized milk with the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Alkaline phosphatase; Standard Minas cheese; Scharer's modified method; Spectrophotometry.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352013000400039
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Novel methods for the encapsulation of meglumine antimoniate into liposomes BJMBR
Frézard,F.; Michalick,M.S.M.; Soares,C.F.; Demicheli,C..
The antimonial drug, meglumine antimoniate, was successfully encapsulated in dehydration-rehydration vesicles and in freeze-dried empty liposomes (FDELs). High encapsulation efficiencies (from 28 to 58%) and low weight ratios of lipids to encapsulated antimony (from 1:0.15 to 1:0.3) were achieved. These formulations, contrary to those obtained by conventional methods, can be stored as intermediate lyophilized forms and reconstituted just before use. The efficacy of FDEL-encapsulated meglumine antimoniate was evaluated in hamsters experimentally infected with Leishmania chagasi. A significant reduction of liver parasite burdens was observed in animals treated with this preparation, when compared to control animals treated with empty liposomes. In contrast,...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Liposomes; Glucantime; Meglumine antimoniate; Leishmaniasis; Encapsulation.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000700016
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