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ปัจจัยต่างๆ ที่มีผลต่อการถ่ายยีนเข้าสู่ปาล์มน้ำมัน Thai Agricultural
Potjamarn Suraninpong; Supawadee Tawaro; Sompong Te-chato.
Oil palm is a highly producing oil plant. The most cultivation area is in the southern part of Thailand. Expended area of oil palm in the others part needs suitable variety. Thus, the object of this study was to improvement of oil palm via gene transfer technique. Embryogenic callus inducing from young leaves of high yield oil palm were cultured on various kinds and concentrations of antibiotics for screening of suitable concentration using for gene transformation. The result showed that hygromycin, kanamycin and phosphinotricin at 20, 200 and 5 mg/l, respectively, were suitable for transformed selection. Cefotaxime concentration at 200 mg/l was suitable for killing of agrobacterium. The study of inoculation time, co-culture time and co-culture...
Tipo: Collection Palavras-chave: Agrobacterium tumefaciens; Oil palm; Gene transfer; Embryogenic callus; Antibiotic; ปาล์มน้ำมัน; การถ่ายยีน; สารปฏิชีวนะ; เอ็มบริโอเจนิคแคลลัส; ระดับความเข้มข้น; แถบดีเอ็นเอ; ใบอ่อน.
Ano: 2011 URL: http://anchan.lib.ku.ac.th/agnet/handle/001/5166
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การประเมินความแปรปรวนของเอ็มบริโอเจนิคแคลลัสปาล์มน้ำมัน ที่เพาะเลี้ยงบนอาหารแข็งและในอาหารเหลวโดยเทคนิค RAPD และ SSR Thai Agricultural
Anchalee Arthipatjaporn; Sompong Te-chato.
Effects of types of medium on somaclonal variation of the embryogenic callus of oil palm wereinvestigated. The calli were cultured either on solidified or in liquidified MS medium supplemented with dicamba at different concentrations and subcultured at monthly intervals for 3 months. The results showed that the maximum growth of the callus at 0.333 gram fresh weight (gFW) and 1.191 gFW were obtained from the solidified and liquidified medium containing 0.1 mg/l dicamba, respectively. Verification of somaclonal variation in embryogenic callus raised on both types of culture media was detected by RAPD and SSR techniques. The RAPD technique revealed that 6 primers namely OPA-03, OPAB-01, OPAB-09, OPAB-14, OPR-11 and OPT-06 gave clear DNA patterns with...
Tipo: Collection Palavras-chave: Oil Palm; SSR techniques; Plant genetics; DNA; RAPD techniques; ปาล์มน้ำมัน; เอ็มบริโอเจนิคแคลลัส; การเพาะเลี้ยงเนื้อเยื่อพืช; พันธุกรรมพืช; เครื่องหมายโมเลกุล; อาหารแข็ง; อาหารเหลว; ความแปรปรวนทางพันธุกรรม.
Ano: 2012 URL: http://anchan.lib.ku.ac.th/agnet/handle/001/5277
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