|
|
|
|
|
| Wang,Jianmin; Song,Ruihua; Chen,Lei; Yin,Liangjun; Chen,Bo; Sun,Jing; Gou,Yuanbing; Zhao,Ling; Chen,Lin. |
| Objective: To investigate the functions of Fibroblast Growth Factor Receptor-2 (FGFR2) at different stages of cell differentiation. The engineered murine embryonic stem (ES) cells with conditional knockout of FGFR2 were developed depending on Cre-loxP. Methods: Cre-loxP system was used in a conditional targeting vector. The competent AM-1 bacteria, which expressed Cre-recombinase, was used to confirm the Cre-mediated deletion of the floxed exons 7 and 8 of FGFR2. The targeting vector was electroporated into the ES cells, and the transfected ES cells were screened with G418 and Ganciclovir. Finally, the ES clones with correct targeting events were identified by Southern Blot and PCR. Results: The targeting vector with conditional knockout of murine FGFR2... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Fibroblast growth factor receptor-2 (FGFR2); Conditional knockout; Homologous recombination; Cre; ES cells. |
| Ano: 2006 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452006000200002 |
| |
|
|
| Li,Kailong; Du,Xiaolan; He,Yani; Zhao,Lin; Yang,Jvrong; Song,Ruihua; Chen,Lin. |
| Objective: To investigate the course of tubular cell senescence and expressions of p53, p21, and Rb during the late phase of ischemia/reperfusion (IRI) in the kidney, and assess the effects of the p53-Rb pathway on tubular cell senescence. Methods: Experimental models of unilateral renal IRI were used in p53(+/+) and p53(-/-) mice. Histological changes at the tubular level, progress of cell senescence, and the expression of Rb, p21, and/or p53 proteins in tubular cells were studied at different moments in time after IRI. Results: Chronic tubulointerstitial fibrosis was much more severe and widely distributed in IRI kidneys of p53(+/+) mice in later stages than in earlier stages. Senescent tubular cells were significantly increased at 3 and 6 months after... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cell senescence; Cell cycle regulatory protein; Ischemia/reperfusion injury; Kidney. |
| Ano: 2007 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452007000200003 |
| |
|
|
|
