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| Gao,W.L.; Wu,L.S.; Zi,J.H.; Wu,B.; Li,Y.Z.; Song,Y.C.; Cai,D.Z.. |
| Although 17β-estradiol (E2) deficiency has been linked to the development of osteoarthritis (OA) in middle-aged women, there are few studies relating other estrogens and estrogen metabolites (EMs) to this condition. We developed a high-performance liquid chromatography-electrospray ionization-tandem mass spectrometry (HPLC-ESI-MS/MS) method to measure the levels of six EMs (i.e., estrone, E2, estriol, 2-hydroxyestrone, 2-hydroxyestradiol, and 16a-hydroxyestrone) in healthy pre- and postmenopausal women and women with OA. This method had a precision ranging from 1.1 to 3.1% and a detection limit ranging from 10 to 15 pg. Compared to healthy women, serum-free E2 was lower in the luteal and postmenopausal phases in women with OA, and total serum E2 was lower... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Estrogen; Estrogen metabolites; Menopause; Serum; Osteoathritis. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000200146 |
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| Shu,H.F.; Gao,F.Y.; Zhang,C.Q.; Liu,S.Y.; Zhang,Z.Y.; Song,Y.C.; Qiu,K.J.; Yang,H.. |
| The molecular mechanisms and potential clinical applications of neural precursor cells have recently been the subject of intensive study. Dlx5, a homeobox transcription factor related to the distal-less gene in Drosophila, was shown to play an important role during forebrain development. The subventricular zone (SVZ) in the adult brain harbors the largest abundance of neural precursors. The anterior SVZ (SVZa) contains the most representative neural precursors in the SVZ. Further research is necessary to elucidate how Dlx5-related genes regulate the differentiation of SVZa neural precursors. Here, we employed immunohistochemistry and molecular biology techniques to study the expression of Dlx5 and related homeobox genes Er81 and Islet1 in neonatal rat... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Neural precursors; Dlx5; Green fluorescent protein; Recombinant plasmids. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000200008 |
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