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Workflows novateurs pour une analyse métabolomique plus claire et plus intégrative ArchiMer
Mondeguer, Florence; Allard, Pierre-marie; Souard, Florence; Elie, Nicolas; Guitton, Yann; Sibat, Manoella; Siano, Raffaele.
During their life cycle, Alexandrium minutum and Scrippsiella donghaienis can produce resistant and revivable cysts that can accumulate in marine sediments for hundreds of years. Our working hypothesis assumes that these two genera have "recorded" their adaptation to ecosystem changes in these cells preserved in the form of cysts. From these revived cysts, toxic and non-toxic dinoflagellate cultures (Alexandrium minutum and Scrippsiella donghaienis) were analyzed by a metabolomic approach to investigate the possible adaptive responses of phytoplankton to these changes in coastal ecosystems. The strains were obtained from isotopically dated sediments, 1986 and 1996 for "old" and 2006 for "modern". In total, 84 samples were obtained. Each experimental...
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Ano: 2019 URL: https://archimer.ifremer.fr/doc/00485/59662/62721.pdf
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Innovative metabolomic workflows to study growth kinetics dinoflagellate cysts revived from modern and ancient sediments ArchiMer
Mondeguer, Florence; Souard, Florence; Guitton, Yann; Allard, Pierre-marie; Elie, Nicolas; Sibat, Manoella; Latimier, Marie; Quere, Julien; Wolfender, Jean-luc; Siano, Raffaele; Hess, Philipp.
Aquatic ecosystems worldwide have been negatively affected by eutrophication, many of them driven by increasing nutrient inputs from untreated domestic sewage and industrial and agricultural wastewater. During their life cycle, Alexandrium minutum and Scrippsiella donghaienis can produce resistant and revivable cysts that can accumulate in the sediments of the coastal marine environment for hundreds of years. As a working hypothesis, we presumed that these two genera have preserved their adaptation to such ecosystem changes in the form of cysts in the old sediments. From revivified cysts, some cultures of toxic and non-toxic dinoflagellates (A. minutum and S. donghaienis) were analyzed by a metabolomic approach to study the possible adaptive responses of...
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Ano: 2018 URL: https://archimer.ifremer.fr/doc/00464/57591/59769.pdf
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First insight on metabolomics workflows and differences between dinoflagellate: characterization and discrimination of modern and ancient strains and their algal growth ArchiMer
Mondeguer, Florence; Souard, Florence; Guitton, Yann; Latimier, Marie; Quere, Julien; Sibat, Manoella; Hess, Philipp; Siano, Raffaele.
Aquatic ecosystems worldwide have been negatively affected by eutrophication, many of them driven by increasing nutrient inputs from untreated domestic sewage and industrial and agricultural wastewater. During their life cycle, Alexandrium minutum and Scrippsiella donghaienis can produce resistant and revivable cysts that can accumulate in the sediments of the coastal marine environment for hundreds of years. As a working hypothesis, we presumed that these two genera have preserved their adaptation to such ecosystem changes in the form of cysts in the old sediments. From revivified cysts, some cultures of toxic and non-toxic dinoflagellates (A. minutum and S. donghaienis) were analyzed by a untargeted metabolomics approach by LC-HRMS to study the possible...
Tipo: Text
Ano: 2018 URL: https://archimer.ifremer.fr/doc/00440/55199/56664.pdf
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Molecular networking on wild “Tali” (Erythrophleum, Fabaceae) from Central tropical Africa ArchiMer
Delporte, C; Vanhaverbeke, C; Noret, N; Van Anterpwen, P; Stévigny, C; Hardy, O; Mondeguer, Florence; Touboul, David; Souard, Florence.
To highlight plant adaptive traits, we have observed the metabolome from leaves of wild type tropical Erythrophleum genus that includes the set of particular metabolites present in an organ at a certain time. To answer those ecological questions, genetic approaches as neutral genetic markers (like microsatellites) are usually used to evaluate how evolutionary forces interact to shape phenotypic variations observed among natural populations. As metabolites are the result of a complex network of gene expression, protein expression and interactions and other regulatory processes, they are therefore closer to the phenotype than transcriptome or proteome. Secondary metabolites resulting from specific biosynthetic pathways possess characteristic structural...
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Ano: 2019 URL: https://archimer.ifremer.fr/doc/00498/60935/64331.pdf
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Sélection de workflows adaptés à une analyse métabolomique plus transparente et intégrative ArchiMer
Mondeguer, Florence; Allard, Pierre-marie; Souard, Florence; Elie, Nicolas; Guitton, Yann; Sibat, Manoella; Siano, Raffaele.
During their life cycle, Alexandrium minutum and Scrippsiella donghaienis can produce resistant and revivable cysts that can accumulate in marine sediments for hundreds of years. Our working hypothesis assumes that these two genera have "recorded" their adaptation to ecosystem changes in these cells preserved in the form of cysts. From these revived cysts, toxic and non-toxic dinoflagellate cultures (Alexandrium minutum and Scrippsiella donghaienis) were analyzed by a metabolomic approach to investigate the possible adaptive responses of phytoplankton to these changes in coastal ecosystems. The strains were obtained from isotopically dated sediments, 1986 and 1996 for "ancient" and 2006 for "modern". In total, 84 samples were obtained. Each experimental...
Tipo: Text
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00498/60941/64339.pdf
Registros recuperados: 5
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