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Reemerging of natural infection by Trypanosoma evansi in horses in Arari, Marajó Island, Brazil Ciência Rural
Silva,Josileide Araújo da; Domiciano,Tarcísio Oliveira; Montão,Daniele Pina; Sousa,Paulo Geovani Silva; Ramos,Leandro Lopes; Paredes,Laura Jamille Argolo; Monteiro,Silvia Gonzalez; Rivero,Gabriela Riet Correa; Scofield,Alessandra; Bezerra Júnior,Pedro; Bezerra,Isis Abel; Cerqueira,Valíria Duarte.
ABSTRACT: Two outbreaks of trypanosomiasis by Trypanosoma evansi in horses in the municipality of Chaves, Marajó Island, Pará State, Brazil are reported. The first outbreak occurred in April 2011 in a farm with 147 horses; of these, 47 (31.97%) got sick, and 40 (27.21%) died. The second outbreak occurred in May 2012 and involved nine properties. Of a total of 679 horses, 209 (30.07%) got sick, and 183 (26.97%) died. The main clinical signs observed in these horses were weight loss, abdominal edema, toe dragging, and pelvic muscle atrophy. Necropsy was performed in two horses, one from each outbreak. Macroscopic alterations were not seen in the horse from the first outbreak; however, the horse from the second outbreak exhibited emaciation, pallor and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Equids; Trypanosomiasis; Pará; Marajó Island.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016001202170
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Molecular detection of Trypanosoma evansi (Kinetoplastida: Trypanosomatidae) in procyonids (Carnivora: Procyonidae) in Eastern Amazon, Brazil Ciência Rural
Magalhães-Matos,Paulo Cesar; Cunha-Santos,Rafaelle; Sousa,Paulo Geovani Silva; Sampaio-Júnior,Francisco Dantas; Barros,Flávia de Nazaré Leite; Mourão,Fábio Rodrigo Paixão; Oliveira,Wanessa Batista Lima; Gabriel,Áurea Martins; Monteiro,Silvia Gonzalez; Góes-Cavalcante,Gustavo; Scofield,Alessandra.
ABSTRACT: The present study aimed to diagnose the natural infection of captive and free-living procyonids with Trypanosoma evansi in the states of Amapá and Pará, Brazil. From February 2012 to August 2013, whole blood samples and blood smears were obtained from 45 free-living procyonids and from nine procyonids kept in captivity in wild life refuges and zoobotanical parks in the states of Amapá and Pará. Whole blood samples were collected and kept at -20ºC for the detection of T. evansi DNA by PCR using the RoTat 1.2 forward and RoTat 1.2 reverse primers. In addition, the blood smears were processed and examined for the presence of trypomastigote forms of T. evansi. T. evansi DNA was detected in 18.52% (10/54) of the procyonids, namely, in captive...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Trypanosoma evansi; PCR; Procyonidae; Eastern Amazon.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000400663
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