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| Souza,A.C.M.F.; Souza,D.R.V.; Sanabani,S.S.; Giorgi,R.R.; Bendit,I.. |
| Amplification of the MYCN gene in neuroblastomas is a potent biological marker of highly aggressive tumors, which are invariably fatal unless sound clinical management is applied. To determine the usefulness of semi-quantitative differential PCR (SQ-PCR) for accurate quantification of MYCN gene copy number, we evaluated the analytical performance of this method by comparing the results obtained with it for 101 tumor samples of neuroblastoma to that obtained by absolute and relative real-time PCR. Similar results were obtained for 100 (99%) samples, no significant difference was detected between the median log10 MYCN copy number (1.53 by SQ-PCR versus 1.55 by absolute real-time PCR), and the results of the two assays correlated closely (r = 0.8, Pearson... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Neuroblastoma; Semi-quantitative PCR; Real-time PCR; MYCN; Amplification. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000900004 |
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