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| Rosa,Daniel D.; Campos,Magnólia A.; Targon,Maria Luisa P.N.; Souza,Alessandra A.. |
| Due to the economic importance of gummosis disease for the citriculture, studies on P. parasitica-Citrus interaction comprise a significant part in the Brazilian Citrus genome data bank (CitEST). Among them, two cDNA libraries constructed from two different growth conditions of the P. parasitica pathogen are included which has generated the PP/CitEST database (CitEST - Center APTA Citros Sylvio Moreira/IAC- Millennium Institute). Through this genomic approach and clustering analyses the following has been observed: out of a total of 13,285 available in the Phytophthora parasitica database, a group of 4,567 clusters was formed, comprising 2,649 singlets and 1,918 contigs. Out of a total of 4,567 possible genes, only 2,651 clusters were categorized; among... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Citrus disease; Elicitins; Plant pathogen; Gene expression profiles. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000500028 |
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| Boscariol-Camargo,Raquel L.; Berger,Irving J.; Souza,Alessandra A.; Amaral,Alexandre M. do; Carlos,Eduardo F.; Freitas-Astúa,Juliana; Takita,Marco A.; Targon,Maria Luisa P.N.; Medina,Camilo L.; Reis,Marcelo S.; Machado,Marcos A.. |
| CitEST project resulted in the construction of cDNA libraries from different Citrus sp. tissues under various physiological conditions. Among them, plantlets of Rangpur lime were exposed to hydroponic conditions with and without water stress using PEG6000. RNA from roots was obtained and generated a total of 4,130 valid cDNA reads, with 2,020 from the non-stressed condition and 2,110 from the stressed set. Bioinformatic analyses measured the frequency of each read in the libraries and yielded an in silico transcriptional profile for each condition. A total of 40 contigs were differentially expressed and allowed to detect up-regulated homologue sequences to well known genes involved in stress response, such as aquaporins, dehydrin, sucrose synthase, and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Expressed sequence tags; Transcription; Rootstock; Drought; Molecular response. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000500019 |
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| Souza,Alessandra A.; Boscariol,Raquel L.; Moon,David H.; Camargo,Luis E.A.; Tsai,Siu M.. |
| Molecular markers were used to estimate the effect of mineral nitrogen on the phenotypic expression of quantitative trait loci (QTL) controlling the number of Rhizobium nodules (NN) and resistance to Xanthomonas axonopodis pv. phaseoli in the common bean. Recombinant inbred lines derived from a BAT-93 x Jalo EEP558 cross were grown in a greenhouse in the absence or presence (5 mM NH4NO3) of nitrogen. Resistance to Xanthomonas was assessed as diseased leaf area (DLA) and the number of nodules was obtained by direct counting. Analyses of variance were used to detect significant associations between 85 marker loci from 12 linkage groups (LG) and quantitative traits. In the absence of nitrogen, 15 and 11 markers, distributed over 7 and 5 LG, showed a... |
| Tipo: Info:eu-repo/semantics/article |
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| Ano: 2000 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000100029 |
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| Fietto,Luciano G.; Costa,Maximiller D.L.; Cruz,Cosme D.; Souza,Alessandra A.; Machado,Marcos A.; Fontes,Elizabeth P.B.. |
| The completion of the genome sequencing of the Arabidopsis thaliana model system provided a powerful molecular tool for comparative analysis of gene families present in the genome of economically relevant plant species. In this investigation, we used the sequences of the Arabidopsis Hsp70 gene family to identify and annotate the Citrus Hsp70 genes represented in the CitEST database. Based on sequence comparison analysis, we identified 18 clusters that were further divided into 5 subgroups encoding four mitochondrial mtHsp70s, three plastid csHsp70s, one ER luminal Hsp70 BiP, two HSP110/SSE-related proteins and eight cytosolic Hsp/Hsc70s. We also analyzed the expression profile by digital Northern of each Hsp70 transcript in different organs and in response... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Chaperones; Digital northern; Heat shock proteins; Genome; Expressed sequence tags. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000500017 |
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| Campos,Magnólia A.; Rosa,Daniel D.; Teixeira,Juliana Érika C.; Targon,Maria Luisa P.N.; Souza,Alessandra A.; Paiva,Luciano V.; Stach-Machado,Dagmar R.; Machado,Marcos A.. |
| In silico expression profiles, of the discovered 3,103 citrus ESTs putatively encoding for PR protein families (PR-1 to PR-17), were evaluated using the Brazil citrus genome EST CitEST/database. Hierarchical clustering was displayed to identify similarities in expression patterns among citrus PR-like gene families (PRlgf) in 33 selected cDNA libraries. In this way, PRlgf preferentially expressed by organ and citrus species, and library conditions were highlighted. Changes in expression profiles of clusters for each of the 17 PRlgf expressed in organs infected by pathogens or drought-stressed citrus species were displayed for relative suppression or induction gene expression in relation to the counterpart control. Overall, few PRlgf showed expression 2-fold... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Citrus; Functional genome; Ests; Gene expression profiles; Pathogenesis-related proteins; Defense genes. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000500020 |
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