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| Oliveira,Micheline de Lucena; Amorim,Melania Maria Ramos de; Souza,Paulo Roberto Eleutério de; Albuquerque,Lúcia Cristina Bezerra de; Brandão,Lucas André Cavalcanti; Guimarães,Rafael Lima. |
| This study compares the detection rates of Chlamydia trachomatis by two techniques, direct immunofluorescence (IMF) and real time polymerase chain reaction (PCR), in patients with and without intra-epithelial cervical lesions (SIL) in Recife. We conducted a transversal study involving 35 women with SIL and 35 without SIL attended at Ambulatório Especializado da Mulher, Recife, Brazil. They were tested for Chlamydia trachomatis using two techniques, direct IMF or real time PCR. The rates of Chlamydia trachomatis detection were compared and the association with intra-epithelial cervical lesions was determined using the chi-square test at a 5% level of significance. Concordance between the tests was evaluated using kappa. The global prevalence of Chlamydia... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Chlamydia trachomatis; Chlamydia infections; Fluorescent antibody technique direct; Polymerase chain reaction. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702008000400014 |
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| Valones,Marcela Agne Alves; Guimarães,Rafael Lima; Brandão,Lucas André Cavalcanti; Souza,Paulo Roberto Eleutério de; Carvalho,Alessandra de Albuquerque Tavares; Crovela,Sergio. |
| Recent developments in molecular methods have revolutionized the detection and characterization of microorganisms in a broad range of medical diagnostic fields, including virology, mycology, parasitology, microbiology and dentistry. Among these methods, Polymerase Chain Reaction (PCR) has generated great benefits and allowed scientific advancements. PCR is an excellent technique for the rapid detection of pathogens, including those difficult to culture. Along with conventional PCR techniques, Real-Time PCR has emerged as a technological innovation and is playing an ever-increasing role in clinical diagnostics and research laboratories. Due to its capacity to generate both qualitative and quantitative results, Real-Time PCR is considered a fast and accurate... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Conventional PCR; Real-Time PCR; Molecular Biology; Methods of molecular detection. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000100001 |
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