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| Dórea,Regina Coeli Cunha; Barboza-Filho,Carlos Gomes; Moreira,Dayson Friaça; Guirro,Mirian; Chaves,Ágtha de Alencar Muniz; Dunder,Ricardo José; Soares,Elaine Amorim; Santos-Júnior,Vitório; Spinosa,Wilma. |
| Little is known on the epitopes derived from metacyclic promastigotes of Leishmania that are important on the regulation or destruction of the parasite, as targets of immune attack in the vertebrate host. In this study we investigated an alternative method to obtain metacyclic promasigotes of Leishmania major, as evaluated by the course of infection and delayed-type hipersensitivity (DTH) in resistant versus susceptible inbred mice. Non-infective (procyclic) promastigotes of L. major recently transformed from tissue amastigotes were attached to a negatively charged glass-wool column, whereas metacyclic promastigotes were not bound to columns and could be easily recovered. Optimal chromatography conditions were validated through statistical analyses.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Leishmania major; Metacyclic promastigotes; Metacyclogenesis; BALB/c mice; C57BL/6 mice. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500006 |
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