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| Spolidorio,Denise Madalena Palomari; Höfling,José Francisco; Pizzolitto,Antônio Carlos; Rosa,Edvaldo Antonio; Negrini,Thaís de Cássia; Spolidorio,Luís Carlos. |
| The aim of this study was to determine whether random amplified polymorphic DNA (AP-PCR) analysis is able to differentiate genetically different clones of mutans streptococci, in 22 Brazilian family members. Stimulated saliva samples were collected from fathers, mothers and infants. For 5-18 months babies with erupting primary dentition, plaque samples were collected using sterile tooth pick tips. From these samples, mutans streptococci were isolated on SB-20 agar plates. After growth, representative colonies were identified by biochemical methods on the basis of carbohydrate fermentation. Streptococcus mutans isolates were obtained from all family members and AP-PCR typed separately with a random primer (OPA-13). Bacterial cell lysates were used as... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Streptococcus mutans; AP-PCR; Genetic polymorphism. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000300006 |
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| Boriollo,Marcelo Fabiano Gomes; Spolidorio,Denise Madalena Palomari; Barros,Letizia Monteiro; Bassi,Rodrigo Carlos; Garcia,José Antonio Dias; Costa,Ana Maria Duarte Dias; Rosa,Edvaldo Antonio Ribeiro; Höfling,José Francisco. |
| The genetic diversity of C. albicans oral isolates from 75 healthy schoolchildren from eight schools located in different geographic areas of Piracicaba city, São Paulo state, Brazil, was established using isoenzymes marker (Multilocus Enzyme Electrophoresis - MLEE) and cluster analysis. Patterns of monoclonal and polyclonal oral colonization by C. albicans within and between groups of schoolchildren were identified. However, significant divergence between the observed and the expected genotypic frequencies (Hardy-Weinberg equilibrium test) was not detected in the geographically adjacent groups, suggesting the hypothesis that populations of healthy schoolchildren do not correspond to the selection factor (differential survival) of strains. Two highly... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Candida albicans; Healthy schoolchildren; Geographic region; MLEE; Cluster analysis. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000300023 |
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