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	Provedor de dados Electron. J. Biotechnol. (1) Autor Madhu,Devaiah (1) Shekar Shetty,Hunthrike (1) Sripathi P,Harishchandra (1) Urs Babitha,Mysore Pandurangaraj (1) Palavra-chave DIBA (1) Downy mildew (1) ELISA (1) Immunofluorescence (1) Manganese superoxide dismutase (1) Pearl millet (1) Sclerospora graminicola (1) Western blot (1) Mais... Tipo do documento Journal article (1) Ano 2006 (1) País Chile (1) Idioma Inglês (1)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 1 Primeira ... 1 ... Última Production of polyclonal antisera to manganese superoxide dismutase expressed in downy mildew resistant pearl millet and its application for immunodiagnosis Electron. J. Biotechnol. Urs Babitha,Mysore Pandurangaraj; Madhu,Devaiah; Sripathi P,Harishchandra; Shekar Shetty,Hunthrike. Level of superoxide dismutase (SOD) in the seedlings of downy mildew resistant pearl millet genotype (IP 18293) increased 3-fold upon inoculation with Sclerospora graminicola. Mn-SOD was purified from pearl millet by ion-exchange and gel filtration chromatography. Polyclonal antibody was raised in rabbit against Mn-SOD of pearl millet. Anti-Mn SOD had an antibody titer of 1:20,000. ELISA results revealed a 1.5-fold increase in pure Mn-SOD preparation compared to the crude preparation. The antibody reactivity as detected by ELISA revealed that Mn-SOD is higher at 4 hrs post-inoculation in resistant pearl millet seedlings. The reactivity also showed the Mn-SOD is more in roots of inoculated resistant seedlings. SOD reactivity was also determined by DIBA... Tipo: Journal article Palavras-chave: DIBA; Downy mildew; ELISA; Immunofluorescence; Manganese superoxide dismutase; Pearl millet; Sclerospora graminicola; Western blot. Ano: 2006 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000200007 Registros recuperados: 1 Primeira ... 1 ... Última

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