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| Vetter,Michele Regina; Staggemeier,Rodrigo; Vecchia,Andréia Dalla; Henzel,Andréia; Rigotto,Caroline; Spilki,Fernando Rosado. |
| Human adenoviruses (HAdV), members of the Adenoviridae family, are excreted through the fecal route and may be present in the feces of humans consuming contaminated food or water. The presence of HAdV from different serotypes in the feces of healthy individuals was already reported using conventional polymerase chain reaction; however, real-time PCR (qPCR) may reveal not only the rates of detection as well as demonstrate the viral loads excreted by healthy persons. Aiming to identify and characterize the presence of adenoviruses in stool samples, 147 fecal samples from patients with no records of diarrhea were analyzed (74 from winter season and 73 from summer) by Real-Time PCR (qPCR) assay and conventional PCR. HAdV genome was present in 43.8% (32/73) of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: HAdV; Stool samples; QPCR; Healthy individuals. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000300749 |
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| Luz,Roger B.; Staggemeier,Rodrigo; Fabres,Rafael B.; Soliman,Mayra C.; Souza,Fernanda G.; Gonçalves,Raoni; Fausto,Ivone V.; Rigotto,Caroline; Heinzelmann,Larissa S.; Henzel,Andréia; Fleck,Juliane D.; Spilki,Fernando R.. |
| Adenoviruses are among the most promising viral markers of fecal contamination. They are frequently found in the water, sediment and soil of regions impacted by human activity. Studies of the bioaccumulation of enteric viruses in shrimp are scarce. The cities located in the northern coast of the lake systems in Southern Brazil have high urbanization and intensive farming rates, and poor sewage collection and treatment. One hundred (n = 100) Farfantepenaeus paulensis pink-shrimp specimens and 48 water samples were collected from coastal lagoons between June 2012 and May 2013. Water samples were concentrated and the shrimp, mashed. After DNA extraction, samples were analyzed by real time polymerase chain reaction (qPCR) in order to detect and quantify viral... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Adenovirus; Farfantepenaeus paulensis; Pink-shrimp; Water quality; Northern Coast of Rio Grande do Sul. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000300715 |
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