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| Cury,Gisele Gentile; Mobilon,Cristiane; Stehling,Eliana Guedes; Lancellotti,Marcelo; Ramos,Marcelo de Carvalho; Martinez,Roberto; Brocchi,Marcelo; Silveira,Wanderley Dias da. |
| One hundred and fifty-one methicillin-resistant z (MRSA) strains have been isolated from patients admitted in tertiary care hospitals in two metropolitan areas (Campinas City and Ribeirão Preto City) in the southeast region of Brazil and analyzed through PCR-based techniques [(PCR amplification of spa, coa, and housekeeping genes (arcC, aroE, gmk, pta, tpi, yqiL)] and further restriction fragment typing of coa and of housekeeping genes. The heterogeneity of spa gene was determined directly by agarose gel electrophoresis migration. The results obtained indicate the existence of three (A, B, C) main clusters. Since the strain distribution in these three clusters is much characteristic, it denotes the existence of three main clones. All strains isolated in... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: MRSA; Nosocomial; Molecular characterization; PCR. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702009000300002 |
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| Lancellotti,Marcelo; Pace,Fernanda de; Stehling,Eliana Guedes; Villares,Maria Cecília Barisson; Brocchi,Marcelo; Silveira,Wanderley Dias da. |
| Forty-five Haemophilus influenzae strains isolated from patients were characterized based on biochemical characteristics. Their capsular types were determined by polymerase chain reaction (PCR); they were compared, using two molecular methods [ribotyping with a specific DNA probe amplified from the 16S rDNA region from H. influenzae and through restriction fragment length polymorphism (RLFP) of an amplified 16S DNA region]. The strains were better discriminated by the ribotyping technique that used the 16S probe and by the combination of both techniques. Biotypes I and IV were the most common, followed by biotypes VI, VIII and III. Biotypes II and VII were not found. Most of the capsular samples were nontypable (89%), with capsular types a and b found in 2... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Haemophilus influenzae; Characterization; Ribotyping; Serotype; Biotype. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702008000500015 |
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| Angelini,Michelle; Stehling,Eliana Guedes; Moretti,Maria Luiza; Silveira,Wanderley Dias da. |
| Shigella spp., the human pathogen responsible for shigellosis, is highly infectious even at low levels. The incidence rate of shigellosis varies with geographical distribution, location human development index, and age groups, being higher among children aged under 5 years. In Brazil, a few works indicate that shigellosis cases are underestimated, with S. flexneri and S. sonnei strains being the major agents responsible for the shigellosis cases. The present study used pulsed field gel electrophoresis (PFGE) to investigate the molecular epidemiology of 119 strains of S. sonnei and S. flexneri isolated from shigellosis cases that occurred in the metropolitan areas of Ribeirão Preto and Campinas Cities, São Paulo Sate, southeast Brazil. The results indicated... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Shigella sonnei; Shigella flexneri; Epidemiology; Characterization; PFGE. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000300034 |
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| Azevedo,Paola Aparecida Alves; Furlan,João Pedro Rueda; Oliveira-Silva,Mariana; Nakamura-Silva,Rafael; Gomes,Carolina Nogueira; Costa,Karen Regina Carim; Stehling,Eliana Guedes; Pitondo-Silva,André. |
| ABSTRACT Enterobacter cloacae and E. aerogenes have been increasingly reported as important opportunistic pathogens. In this study, a high prevalence of multi-drug resistant isolates from Brazil, harboring several β-lactamase encoding genes was found. Several virulence genes were observed in E. aerogenes, contrasting with the E. cloacae isolates which presented none. |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Enterobacter cloacae; Enterobacter aerogenes; Virulence; Resistance. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000500224 |
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