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	Provedor de dados PAB (2) Autor Bona,Claudine Maria de (2) Louzada,Eliezer Silva (2) Stelly,David (2) Gould,Jean Howe (1) Miller Jr,J. Creighton (1) Miller Jr.,J. Creighton (1) Mais... Palavra-chave Asymmetric somatic hybridization (1) Citrus (1) Citrus sp. (1) Gamma irradiation (1) Gamma rays (1) Grapefruit (1) Hybrid cell lines (1) Protoplast fusion (1) Radiation hybrid mapping (1) Somatic hybridization (1) Sweet orange (1) Swinglea glutinosa (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2009 (2) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Fusion of protoplasts with irradiated microprotoplasts as a tool for radiation hybrid panel in citrus PAB Bona,Claudine Maria de; Stelly,David; Miller Jr.,J. Creighton; Louzada,Eliezer Silva. The objective of this work was to combine asymmetric somatic hybridization (donor-recipient fusion or gamma fusion) to microprotoplast-mediated chromosome transfer, as a tool to be used for chromosome mapping in Citrus. Swinglea glutinosa microprotoplasts were irradiated either with 50, 70, 100 or 200 gamma rays and fused to cv. Ruby Red grapefruit or Murcott tangor protoplasts. Cell colonies were successfully formed and AFLP analyses confirmed presence of S. glutinosa in both 'Murcott' tangor and 'Ruby Red' grapefruit genomes. Tipo: Info:eu-repo/semantics/article Palavras-chave: Citrus; Swinglea glutinosa; Asymmetric somatic hybridization; Gamma rays; Hybrid cell lines; Radiation hybrid mapping. Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2009001200008 Citrus asymmetric somatic hybrids produced via fusion of gamma-irradiated and iodoacetamide-treated protoplasts PAB Bona,Claudine Maria de; Gould,Jean Howe; Miller Jr,J. Creighton; Stelly,David; Louzada,Eliezer Silva. The objective of this study was to produce citrus somatic asymmetric hybrids by fusing gamma-irradiated protoplasts with iodoacetamide-treated protoplasts. Protoplasts were isolated from embryogenic suspension cells of grapefruit (Citrus paradisi Macfad.) cultivars Ruby Red and Flame, sweet oranges (C. sinensis Osbeck) 'Itaboraí', 'Natal', Valencia', and 'Succari', from 'Satsuma' (C. unshiu Marcow.) and 'Changsha' mandarin (C. reticulata Blanco) and 'Murcott' tangor (C. reticulata x C. sinensis). Donor protoplasts were exposed to gamma rays and receptor protoplasts were treated with 3 mmol L-1 iodoacetamide (IOA), and then they were fused for asymmetric hybridization. Asymmetric embryos were germinated, and the resulting shoots were either grafted onto... Tipo: Info:eu-repo/semantics/article Palavras-chave: Citrus sp.; Gamma irradiation; Grapefruit; Protoplast fusion; Somatic hybridization; Sweet orange. Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2009000500004 Registros recuperados: 2 Primeira ... 1 ... Última

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