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| González-Cabrera,Jaime; Arredondo-Bernal,Hugo C.; Stouthamer,Richard. |
| Accurate identification of species as biological candidates is the first step in establishing successful biological control programs. In Mexico, Trichogramma parasitoids are identified using morphological characters, which is a laborious procedure made only by experts; in addition, some morphological structures are susceptible to changes due to differences in diet or environment, and consequently there is a latent risk of misidentification. Moreover, morphological identification requires the presence of males, leaving female-only populations unidentifiable. As an alternative to morphological identification, the purpose of this work was to develop a molecular method for the identification of Trichogramma parasitoids from Mexican insectaries. Trichogramma... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Trichogramma pretiosum; T. exiguum; T platneri; T. fuentesi; DNA-identification; ITS2 sequences. |
| Ano: 2014 |
URL: http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S1405-31952014000700004 |
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| Almeida,Raul P. de; Stouthamer,Richard. |
| Discovery of male morphological characters for species identification was a great improvement in Trichogramma systematic. However, not all species could be easily identified. In some cases, the lack of males (thelytokous status of species that carry the Wolbachia symbiont) made Trichogramma identification impossible. This problem was solved via antibiotic and heating treatments for elimination of the bacteria and allowing the production of males. The only Trichogramma species reported in which thelytoky is not induced by bacterial infection is T. cacoeciae, so here another means of species identification is needed. This species was identified based on the ITS2 (Internal transcribed spacer 2) sequence, a modern technique that has been proved useful in... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Insecta; Parasitoid; Thelytoky; Ribosomal DNA. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2003000200011 |
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