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| Pereira,M.A.A.; Nowosh,V.; Suffys,P.N.; Queiroz,G.B.; Silva,K.M.O.; Lourenço,M.C.S.; Vicente,A.C.P.; Fontes,A.N.B.; Morgado,S.; Neves,R.C.S.M.. |
| ABSTRACT Canine Leproid Granuloma Syndrome (CLGS), also known as canine leprosy, is a cutaneous nodular infectious disease caused by Mycobacterium sp.. Despite being reported worldwide, it is still quite unknown and underdiagnosed. Diagnosis may be achieved by cytopathology or histopathology of skin lesions, but identification of the infectious agent is complex, since bacterial in vitro growth is not possible, relying upon molecular techniques such as PCR to confirm Mycobacterium DNA in the sample. We report a CLGS case in Niteroi, Rio de Janeiro state, Brazil, diagnosed by cytopathology and submitted to molecular identification of the agent. PCR amplification of hsp65 gene was performed and revealed 100% genetic homology to M. murphy strain. This is the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: CLGS; Hsp65 sequence; Isolate Metcalfe; Molecular methods. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352018000601699 |
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| Ivens-de-Araujo,M.E.; Fandinho,F.C.; Werneck-Barreto,A.M.; Gonçalves-Veloso,V.; Grinstejn,B.; Lourenço,M.K.; Werneck-Barroso,E.; Degrave,W.M.; Suffys,P.N.. |
| Isolates of Mycobacterium tuberculosis derived from patients with AIDS from a single hospital in Rio de Janeiro were typed using a standardized RFLP technique detecting IS6110 polymorphism. Nineteen isolates were obtained from 15 different patients. Eleven distinct IS6110 patterns were found, with 4 banding patterns shared by 2 patients. The clustering value of 53% was much higher in comparison with clustering of M. tuberculosis strains from TB patients without clinical signs for HIV infection from randomly selected health centers. We present these results as preliminary data on M. tuberculosis strain polymorphism in Brazil and on the higher risk for recent transmission amongst patients with AIDS |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Tuberculosis; Fingerprinting; AIDS. |
| Ano: 1998 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000300006 |
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