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Comparison of isozyme transformation in maize as a result of insertion of the chitinase gene Phyton
Yan,PM; Zhang,HF; Wang,Q; Yan,XY; Sun,Y.
Isozymes of peroxidase (POD), catalase (CAT), esterase (EST) and superoxide dismutase (SOD) were analyzed on transgenic maize (with external chitinase gene) and its parent by vertical polyacrylamide gel electrophoresis (PAGE). This study was made using shoots at the fourth leaf stage. Results showed that: POD and EST were detected in 6 bands. POD-2 and POD-3 were present at the bud and seedling stages. POD-1, POD-4, POD-5 and POD-6 were only present at the seedling stage. POD-6 expressed stronger in the transgenic maize with chitinase than in its parent. EST-2 was present only at the bud stage, and its expression in transgenic maize was stronger than that in its parent. EST-5 only existed at the seedling stage. EST-4 did not exist in the parent maize...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Chitinase; Isozyme; Maize; Antioxidant enzyme system.
Ano: 2010 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-56572010000200002
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Silicon-mediated alleviation of Cadmium toxicity on Tujopsis dolabrata Phyton
Huang,YC; Chen,H; Zhao,WJ; Li,WD; Yang,HY; Sun,Y; Wang,L; Cao,SH.
We conducted pot experiments on the cypress Thujopsis dolabrata (Linn. f.) Sieb. et Zucc. in order to study the interaction of silicon (Si) and root exudates on cadmium (Cd) bioavailability in the rhizosphere,. Each variety was planted with 100 mg/kg Cd and/or 400 mg/kg Si for 210 days. The results showed that adding Si increased Cd tolerance in T. dolabrata, but that the mechanism was specifical. In T. dolabrata, Si did not prevent Cd translocation from roots to shoots, and it significantly enhanced Cd accumulation without inhibiting growth. Moreover, Si mobilized Cd from the rhizospheric soil by stimulating phenolic exudation from the roots, suggesting that Cd-chelation combined with Si-induced phenolics were involved in Cd detoxification.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cadmium; Rhizosphere; Root exudation; Silicon; Thujopsis dolabrata.
Ano: 2016 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-56572016000200016
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Total aboveground plant biomass is more strongly affected by climate than species diversity on a grassland in Liaoning, China Phyton
Zhou,C; Busso,C; Liu,J; Yang,YG; Sun,Y; Fang,YZ; Zhang,QQ; Zhou,YB; Wang,YN; Zhang,Z; Wang,ZW; Yang,YF.
The objective of this study was to analyze the relationships between total aboveground plant biomass versus altitude, latitude, longitude, mean annual temperature, mean annual precipitation, and species diversity. Simple linear regression analysis was used to study these relationships. Results showed that altitude was significantly, positively (R² = 0.038, P<0.01) related with total plant aboveground biomass. Meanwhile, when longitude (R² = 0.124, P<0.001) and latitude (R² = 0.221, P<0.001) increased, total aboveground biomass decreased. The relationship between biomass and mean annual precipitation was significantly, positively linear (R² = 0.149, P<0.001). Mean annual temperature was signifcantly, negatively correlated with biomass (R² =...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Productivity; Species diversity; Climate; Grasslands.
Ano: 2016 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-56572016000100017
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The Expression of Can and Camk is Associated with Lipogenesis in the Muscle of Chicken Rev. Bras. Ciênc. Avic.
Yang,Y; Song,J; Fu,R; Sun,Y; Wen,J.
ABSTRACTIntramuscular fat (IMF) content in chickens significantly contributes to meat quality. The main objective of this study was to assess the expression of calcineurin (CaN) and Ca2+/calmodulin-dependent protein kinase (CaMK) in lipogenesis in chicken muscle. Chickens were slaughtered and sampled at 4, 8, and 16 weeks of age. IMF content and the expression of CaN subunits and CaMK isoforms were measured in the thigh muscle tissue. The results showed that the IMF contents were greater at 16 weeks compared with those at 4 and 8 weeks (p<0.05). Transcription of fatty acid synthase (FAS) and fatty acid translocase CD36 (FAT/CD36) mRNA significantly increased with age, from four to 16 weeks (p<0.05). The mRNA levels of CaNB and CaMK IV were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ca2+/calmodulin-dependent protein kinase; Calcineurin; Chicken; Regulation..
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2015000300287
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