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	Provedor de dados 17 (1) Autor Amit Shavit (1) Susan C. Roberts (1) Vishal Gaurav (1) Palavra-chave Biotechnology (1) Cancer (1) Developmental Biology (1) Genetics & Genomics (1) Plant Biology (1) Tipo do documento Poster (1) Ano 2009 (1) País United Kingdom (1)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 1 Primeira ... 1 ... Última Nuclear DNA and protein content evaluation in Taxus plant cell cultures using multiparameter flow cytometry 17 Vishal Gaurav; Amit Shavit; Susan C. Roberts. Plant cell cultures of Taxus provide the most reliable production methods for the anti-cancer drug paclitaxel. In order to comprehend the inherent culture heterogeneity and production variability in cell cultures, it is essential that the cellular metabolism is studied at the genomic level. Genomic stability in plant cell cultures is crucial as it affects cell growth and division, metabolite accumulation and protein synthesis. A rapid and efficient method to prepare nuclei suspensions from aggregated cell cultures of Taxus was employed. Methods were subsequently developed to simultaneously stain them for DNA and protein content using Propidium Iodide and Fluorescein Isothiocyanate respectively. Flow cytometry was used to analyze and quantify the DNA... Tipo: Poster Palavras-chave: Biotechnology; Cancer; Developmental Biology; Genetics & Genomics; Plant Biology. Ano: 2009 URL: http://precedings.nature.com/documents/3809/version/1 Registros recuperados: 1 Primeira ... 1 ... Última

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