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Effects of silk sericin on the proliferation and apoptosis of colon cancer cells Biol. Res.
Kaewkorn,Waraporn; Limpeanchob,Nanteetip; Tiyaboonchai,Waree; Pongcharoen,Sutatip; Sutheerawattananonda,Manote.
Sericin is a silk protein woven from silkworm cocoons (Bombyx mori). In animal model, sericin has been reported to have anti-tumoral action against colon cancer. The mechanisms underlying the activity of sericin against cancer cells are not fully understood. The present study investigated the effects of sericin on human colorectal cancer SW480 cells compared to normal colonic mucosal FHC cells. Since the size of the sericin protein may be important for its activity, two ranges of molecular weight were tested. Sericin was found to decrease SW480 and FHC cell viability. The small sericin had higher anti-proliferative effects than that of the large sericin in both cell types. Increased apoptosis of SW480 cells is associated with increased caspase-3 activity...
Tipo: Journal article Palavras-chave: Silk sericin; Colon cancer; Apoptosis; Bcl-2; Caspase-3.
Ano: 2012 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602012000100006
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Protective effect of silk lutein on ultraviolet B-irradiated human keratinocytes Biol. Res.
Pongcharoen,Sutatip; Warnnissorn,Prateep; Lertkajornsin,Ongart; Limpeanchob,Nanteetip; Sutheerawattananonda,Manote.
Carotenoids are efficient antioxidants that are of great importance for human health. Lutein and zeaxanthin are carotinoids present in high concentrations in the human retina which are involved in the photoprotection of the human eye. Lutein may also protect the skin from ultraviolet (UV)-induced damage. The present study investigated the protective effect of lutein extracted from yellow silk cocoons of Bombyx mori on human keratinocytes against UVB irradiation. A human keratinocyte cell line and primary human keratinocytes were used to investigate the UVB protection effects of silk lutein and plant lutein. Silk lutein showed no cytotoxicity to keratinocytes. Treatment with silk lutein prior to UVB irradiation enhanced cell viability and cell...
Tipo: Journal article Palavras-chave: Silk lutein; Keratinocytes; UV; Apoptosis.
Ano: 2013 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000100006
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Immune enhancement activities of silk lutein extract from Bombyx mori cocoons Biol. Res.
Promphet,Porkaew; Bunarsa,Sirirat; Sutheerawattananonda,Manote; Kunthalert,Duangkamol.
BACKGROUND: Declining immune function poses an important clinical challenge worldwide and supplementation with natural products that possessing immune enhancing properties is a promising approach for preventing or delaying immune function decline. Cocoons from yellow silkworms are a significant source of lutein, and this unexplored silk extract could be a viable alternative source for dietary lutein. This study assessed immunomodulatory activities of the silk lutein extract. Female BALB/c mice orally received lutein, either as silk or marigold extracts (10 or 20 mg/kg daily), or vehicle only (1% tween 80 in PBS pH 7.4) for 4 weeks. Natural killer (NK) cell activity, specific antibody production, lymphocyte subpopulations, mitogen-induced lymphocyte...
Tipo: Journal article Palavras-chave: Antibody production; BALB/c mice; Immune function; Interleukin-2; Natural killer cells; Silk lutein extract.
Ano: 2014 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100015
Registros recuperados: 3
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