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| 竹内, 宏治; 井熊, 武志; 高橋, 祐司; 匂坂, 慶子; 髙澤, 俊英; TAKEUCHI, Kouji; IKUMA, Takeshi; TAKAHASHI, Yuji; SAGISAKA, Keiko; TAKASAWA, Toshihide. |
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Palavras-chave: フェノール-硫酸法; 高感度フェノール-硫酸法; 全糖定量法. |
| Ano: 2001 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/1253 |
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| 高橋, 裕司; 井熊, 武志; 匂坂, 慶子; 斉藤, 泉; 髙澤, 俊英; TAKAHASHI, Yuji; IKUMA, Takeshi; SAGISAKA, Keiko; SAITO, Izumi; TAKASAWA, Toshihide. |
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Palavras-chave: Sclerotinia borealis; 好冷菌; 雪腐菌核病菌; ポリガラクツロナーゼ; 細胞壁分解酵素; Psychrophile; Snow mold; Cell wall degrading enzyme; Polygalacturonase. |
| Ano: 2002 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/120 |
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| 井熊, 武志; 竹内, 宏治; 高橋, 祐司; 匂坂, 慶子; 高澤, 俊英; IKUMA, Takeshi; TAKEUCHI, Kouji; TAKAHASHI, Yuji; SAGISAKA, Keiko; TAKASAWA, Toshihide. |
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Palavras-chave: 還元糖定量法; 高感度定量法; 鉄試薬比色定量法; Reducing sugar determination; High sensitive determination; Colorimetric determination. |
| Ano: 2001 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/1257 |
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| 池浦, 真奈美; 波川, 啓土; 高橋, 裕司; 匂坂, 慶子; 髙澤, 俊英; IKEURA, Manami; NAMIKAWA, Yoshitada; TAKAHASHI, Yuji; SAGISAKA, Keiko; TAKASAWA, Toshihide. |
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Palavras-chave: 還元糖定量法; 高感度定量法; 鉄試薬比色定量法; Reducing sugar determination; High sensitive determination; Colorimetric determination. |
| Ano: 2003 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/112 |
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| TAKAHASHI, Yuji; AKAI, Makoto; MURASE, Harutaka; NAMBO, Yasuo. |
| The objective of the present study was to verify the seasonal luteal activity of racehorses in training in Japan from March to August. We allocated 102 horses into a luteal activity group and non-luteal activity group. The luteal activity group included horses with serum progesterone levels that were consistently >1 ng/ml and changed by ± 1 ng/ml. In contrast, the progesterone levels of the non-luteal activity group were consistently <1 ng/ml. In late spring (from May 1 to June 30) and summer (from July 1 to August 31), the percentage of horses in the luteal activity group was significantly higher than in early spring (from March 1 to April 30, P<0.01). These findings demonstrate clear seasonal variations in ovarian activity. The present study... |
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Palavras-chave: Racehorse; Progesterone; Seasonal change. |
| Ano: 2015 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4290 |
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| 高橋, 裕司; 井熊, 武志; 匂坂, 慶子; 斉藤, 泉; 髙澤, 俊英; TAKAHASHI, Yuji; IKUMA, Takeshi; SAGISAKA, Keiko; SAITO, Izumi; TAKASAWA, Toshihide. |
| CM-Toyopearl陽イオン交換カラムクロマトグラフィーおよびSephacryl S-200ゲルろ過カラ ムクロマトグラフィーによって、常温性菌核病菌Sclerotinia sclerotiorumの20℃培養抽出物 のポリガラクツロナーゼ活性画分からポリガラクツロナーゼⅠを単離した。ポリガラクツ ロナーゼⅠ精製標品(P4-4-2)の比活性は3 662U/mgであり、粗抽出液から全体として116倍に 精製した。精製標品はポリアクリルアミドゲル電気泳動によって、均一であることが示さ れた。また、SDS存在下ポリアクリルアミドゲル電気泳動によって本酵素の分子質量は 39.8±1.10kDa(S.E.)、さらに等電点電気泳動によって本酵素の等電点はpH8.70と見積もられた。 Polygaracturonasehas been isolated from the mesophilic white mold Sclerotinia Sclerotiorum by cation-exchange chromatography on a column of CM-Toyopearl followed by gel filtration on a column of Sephacryl S-200. Purified enzyme preparation(P4-4-2) had a specific activity of 3 662 units per mg of protein, that is, 116-fold purification over the crude extract of the wheat bran culture of the mesophilic fungus S.... |
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Palavras-chave: Sclerotinia sclerotiorum; 常温菌; 菌核病菌; ポリガラクツロナーゼ; 細胞壁分解酵素; Mesophile; White mold; Polygalacturonase; Cell wall degrading enzyme. |
| Ano: 2002 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/106 |
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