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EST sequencing and SSR marker development from cultivated peanut (Arachis hypogaea L.) Electron. J. Biotechnol.
Song,Guo Qi; Li,Meng Jun; Xiao,Han; Wang,Xing Jun; Tang,Rong Hua; Xia,Han; Zhao,Chuan Zhi; Bi,Yu Ping.
Making use of the gene resources of wild type peanuts is a way to increase the genetic diversity of the cultivars. Marker assisted selection (MAS) could shorten the process of inter-specific hybridization and provide a possible way to remove the undesirable traits. However, the limited number of molecular markers available in peanut retarded its MAS process. We started a peanut ESTs (Expressed Sequence Tags) project aiming at cloning genes with agronomic importance and developing molecular markers. In this study we found 610 ESTs that contained one or more SSRs from 12,000 peanut ESTs. The most abundant SSRs in peanut are trinucleotides (66.3%) SSRs and followed by dinucleotide (28.8%) SSRs. AG/TC (10.7%) repeat was the most abundant and followed by CT/GA...
Tipo: Journal article Palavras-chave: Arachis hypogaea L.; Cultivars; EST-SSR; Polymorphism; Wild-type peanut.
Ano: 2010 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000300007
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