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	Provedor de dados Electron. J. Biotechnol. (1) Autor Pan,Siyi (1) Tang,Xixuan (1) Wang,Lufeng (1) Wu,Ting (1) Zhou,Ying (1) Palavra-chave &#946 (1) -glucosidase characters chitosan beads immobilization response surface methodology (1) Tipo do documento Journal article (1) Ano 2013 (1) País Chile (1) Idioma Inglês (1)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 1 Primeira ... 1 ... Última Optimal immobilization of β-glucosidase into chitosan beads using response surface methodology Electron. J. Biotechnol. Zhou,Ying; Wang,Lufeng; Wu,Ting; Tang,Xixuan; Pan,Siyi. Background: β-Glucosidase is known as an effective catalyst for the hydrolysis of various glycosides and immobilization is one of the most efficient strategies to improve its activity recovery and properties. Results: Crosslinking-adsorption-crosslinking method was employed to immobilize β-glucosidase into chitosan beads and response surface methodology (RSM) was used to optimize the immobilized conditions of the maximum activity recovery. Enzyme concentration and adsorption time were found to be significant influence factors, and the maximum activity recovery (50.75%) obtained from response surface methodology was in excellent agreement with experimental value (50.81%). Furthermore, various characteristics of immobilized... Tipo: Journal article Palavras-chave: β -glucosidase characters chitosan beads immobilization response surface methodology. Ano: 2013 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000600006 Registros recuperados: 1 Primeira ... 1 ... Última

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