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	Provedor de dados BJM (2) Ciência Rural (2) Autor Taniwaki,Sueli Akemi (4) Araújo Júnior,João Pessoa (2) Brandão,Paulo (1) Brandão,Paulo Eduardo (1) Filoni,Claudia (1) Galdino,Maicon Vinícius (1) Hagiwara,Mitika Kuribayashi (1) Heinemann,Marcos Bryan (1) Hora,Aline Santana da (1) Kurissio,Jacqueline Kazue (1) Lara,Valéria Maria (1) Miotto,Bruno Alonso (1) Ono,Ekaterina Alexandrovna Durymanova (1) Rodrigues,Marianna Vaz (1) Zanutto,Marcelo de Souza (1) Mais... Palavra-chave Cats (1) Diagnosis (1) Dog (1) Epidemiology (1) FIV (1) Feline immunodeficiency virus (1) Feline pathogen (1) Gammaherpesvirus (1) Herpesvirus (1) Infectious diseases (1) Leptospirosis (1) Lipl32 (1) QPCR (1) RNA interference (1) Rabies (1) Risk factors (1) Treatment (1) Urine (1) Mais... Tipo do documento Info:eu-repo/semantics/article (4) Ano 2018 (2) 2017 (1) 2008 (1) País Brazil (4) Idioma Inglês (4)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 4 Primeira ... 1 ... Última Short interfering RNAs targeting a vampire-bat related rabies virus phosphoprotein mRNA BJM Ono,Ekaterina Alexandrovna Durymanova; Taniwaki,Sueli Akemi; Brandão,Paulo. Abstract The aim of this study was to assess the in vitro and in vivo effects of short-interfering RNAs (siRNAs) against rabies virus phosphoprotein (P) mRNA in a post-infection treatment for rabies as an extension of a previous report (Braz J Microbiol. 2013 Nov 15;44(3):879-82). To this end, rabies virus strain RABV-4005 (related to the Desmodus rotundus vampire bat) were used to inoculate BHK-21 cells and mice, and the transfection with each of the siRNAs was made with Lipofectamine-2000™. In vitro results showed that siRNA 360 was able to inhibit the replication of strain RABV-4005 with a 1 log decrease in virus titter and 5.16-fold reduction in P mRNA, 24 h post-inoculation when compared to non-treated cells. In vivo, siRNA 360 was able to induce... Tipo: Info:eu-repo/semantics/article Palavras-chave: Rabies; Treatment; RNA interference. Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000300566 Development and validation of a modified TaqMan based real-time PCR assay targeting the lipl32 gene for detection of pathogenic Leptospira in canine urine samples BJM Miotto,Bruno Alonso; Hora,Aline Santana da; Taniwaki,Sueli Akemi; Brandão,Paulo Eduardo; Heinemann,Marcos Bryan; Hagiwara,Mitika Kuribayashi. Abstract A modified TaqMan real-time polymerase chain reaction targeting a 138 bp fragment within the lipl32 gene was developed to identify exclusively pathogenic Leptospira spp. in dog urine samples. Thirty-five samples from dogs with suspected clinical leptospirosis and 116 samples from apparently healthy dogs were tested for presence of leptospiral DNA using the TaqMan-based assay. The results were compared with those from a well-established conventional PCR targeting the 16S RNA encoding gene associated with nucleotide sequencing analysis. The overall agreement between the assays was 94.8% (confidence interval [CI] 95% 88-100%). The newly developed assay presented 91.6% (CI 95% 71.5-98.5%) relative sensitivity (22[+] lipl32 PCR/24[+] 16S RNA and... Tipo: Info:eu-repo/semantics/article Palavras-chave: Leptospirosis; Dog; Urine; QPCR; Lipl32. Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000300584 Felis catus gammaherpesvirus 1 (FcaGHV1) and coinfections with feline viral pathogens in domestic cats in Brazil Ciência Rural Kurissio,Jacqueline Kazue; Rodrigues,Marianna Vaz; Taniwaki,Sueli Akemi; Zanutto,Marcelo de Souza; Filoni,Claudia; Galdino,Maicon Vinícius; Araújo Júnior,João Pessoa. ABSTRACT: Felis catus gammaherpesvirus 1 (FcaGHV1) may causes an asymptomatic infection that result in an efficient transmission and subsequently dissemination of the virus in feline population. This study used molecular detection by qPCR (quantitative PCR) based on DNA polymerase gene fragment amplification to evaluate the occurrence of FcaGHV1 and its correlation with other feline viral pathogens, such as Carnivore protoparvovirus 1 (CPPV-1), Felid alphaherpesvirus 1 (FeHV-1), and feline retroviruses such as feline immunodeficiency virus (FIV) and feline leukemia virus (FeLV). Of the 182 blood samples evaluated 23.6% (43/182) were positives for FcaGHV1. Approximately 37.9% (33/87) of the samples that tested positive for retrovirus were also were positive... Tipo: Info:eu-repo/semantics/article Palavras-chave: Gammaherpesvirus; Herpesvirus; Feline pathogen; Infectious diseases; Diagnosis. Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000300451 Occurrence of feline immunodeficiency virus infection in cats Ciência Rural Lara,Valéria Maria; Taniwaki,Sueli Akemi; Araújo Júnior,João Pessoa. The occurrence of feline immunodeficiency virus (FIV) in Brazil has been previously described. This study aimed to investigate the frequency of FIV infection in 454 blood samples from healthy and sick domestic cats from 13 cities of São Paulo State, Brazil as well as to evaluate the risk factors associated with the infection. The results showed that 14.7% (67/454) of the cats were infected with FIV. The clinical evaluation showed that 29.2% of the FIV-positive animals were sick, while 7.3% did not show any type of clinical manifestation. In addition, the vast majority (23.1%) of positive cases corresponded to free-roaming owned cats. The incidence of FIV infection was higher in males (20.3%) than in females (9.7%). The results suggest that certain... Tipo: Info:eu-repo/semantics/article Palavras-chave: Feline immunodeficiency virus; Epidemiology; Cats; FIV; Risk factors. Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782008000800024 Registros recuperados: 4 Primeira ... 1 ... Última

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