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	Provedor de dados BJMBR (2) Autor Fernandez,R. (2) Tararthuch,A.L. (2) Biscaia,S.M.P. (1) Coutinho,A.G.G. (1) Malnic,G. (1) Palavra-chave ATP (1) CAMP (1) Cell pH (1) Cl- (1) Cytotoxicity (1) Gentamicin (1) H+-ATPase (1) MDCK-C11 cells (1) Valinomycin (1) [Ca2+]i (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2018 (1) 2007 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Cl- and regulation of pH by MDCK-C11 cells BJMBR Tararthuch,A.L.; Fernandez,R.; Malnic,G.. The interaction between H+ extrusion via H+-ATPase and Cl- conductance was studied in the C11 clone of MDCK cells, akin to the intercalated cells of the collecting duct. Cell pH (pHi) was measured by fluorescence microscopy using the fluorescein-derived probe BCECF-AM. Control recovery rate measured after a 20 mM NH4Cl acid pulse was 0.136 ± 0.008 pH units/min (dpHi/dt) in Na+ Ringer and 0.032 ± 0.003 in the absence of Na+ (0 Na+). With 0 Na+ plus the Cl- channel inhibitor NPPB (10 µM), recovery was reduced to 0.014 ± 0.001 dpHi/dt. 8-Br-cAMP, known to activate CFTR Cl- channels, increased dpHi/dt in 0 Na+ to 0.061 ± 0.009 and also in the presence of 46 nM concanamycin and 50 µM Schering 28080. Since it is thought that the Cl- dependence of H+-ATPase might... Tipo: Info:eu-repo/semantics/article Palavras-chave: Cell pH; CAMP; ATP; H+-ATPase; Valinomycin; Cl-. Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000500012 The aminoglycoside antibiotic gentamicin is able to alter metabolic activity and morphology of MDCK-C11 cells: a cell model of intercalated cells BJMBR Coutinho,A.G.G.; Biscaia,S.M.P.; Fernandez,R.; Tararthuch,A.L.. It is well known that the aminoglycoside antibiotic gentamicin is capable of causing damage to kidney cells. Given the known involvement of Ca2+ in the nephrotoxic action of gentamicin, the purpose of this study was to establish a relationship between the concentration of intracellular Ca2+ ([Ca2+]i) and cellular cytotoxicity using MDCK-C11 cells, a clone that has several properties that resemble those of intercalated cells of the distal nephron. Changes in [Ca2+]i was determined using fluorescence microscopy. Cell viability was evaluated by the neutral red method, and cell cytotoxicity by the MTT method. The [Ca2+]i gradually increased when cells were exposed to 0.1 mM gentamicin for 10, 20, and 30 min. The presence of extracellular Ca2+ was found to be... Tipo: Info:eu-repo/semantics/article Palavras-chave: Gentamicin; [Ca2+]i; MDCK-C11 cells; Cytotoxicity. Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018001000610 Registros recuperados: 2 Primeira ... 1 ... Última

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