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	Provedor de dados Electron. J. Biotechnol. (1) PAB (1) Autor Taski-Ajdukovic,Ksenija (2) Curcic,Zivko (1) Danojevic,Dario (1) Djordjevic,Vuk (1) Kovacev,Lazar (1) Nagl,Nevena (1) Vidic,Milos (1) Vujakovic,Milka (1) Mais... Palavra-chave Beta vulgaris (1) Drought (1) Glycine max (1) Glycinin (1) In vitro (1) Protein content (1) Real-time PCR (1) Subunit composition (1) Β-conglycinin (1) Mais... Tipo do documento Info:eu-repo/semantics/article (1) Journal article (1) Ano 2012 (1) 2010 (1) País Brazil (1) Chile (1) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Development and application of qRT-PCR for sugar beet gene expression analysis in response to in vitro induced water deficit Electron. J. Biotechnol. Taski-Ajdukovic,Ksenija; Nagl,Nevena; Kovacev,Lazar; Curcic,Zivko; Danojevic,Dario. Sugar beet is a significant industrial crop, often grown in the areas where summer drought can severely limit root yield and sugar content. In order to improve development of sugar beet cultivars with increased drought tolerance it is necessary to understand plant response to water stress at the genomic level. Since recent research efforts have focused on the molecular response of the plant in order to identify water deficit inducible genes, the aim of this investigation was to develop qRT-PCR methodology for the quantification of gene expression in sugar beet under conditions of water deficiency in vitro. Sugar beet genotypes, selected for different response to water deficit, were grown and multiplied in vitro. Axilary shoots were placed on... Tipo: Journal article Palavras-chave: Beta vulgaris; Drought; In vitro; Real-time PCR. Ano: 2012 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000600010 Subunit composition of seed storage proteins in high-protein soybean genotypes PAB Taski-Ajdukovic,Ksenija; Djordjevic,Vuk; Vidic,Milos; Vujakovic,Milka. The objective of this work was to quantify the accumulation of the major seed storage protein subunits, β-conglycinin and glycinin, and how they influence yield and protein and oil contents in high-protein soybean genotypes. The relative accumulation of subunits was calculated by scanning SDS-PAGE gels using densitometry. The protein content of the tested genotypes was higher than control cultivar in the same maturity group. Several genotypes with improved protein content and with unchanged yield or oil content were developed as a result of new breeding initiatives. This research confirmed that high-protein cultivars accumulate higher amounts of glycinin and β-conglycinin. Genotypes KO5427, KO5428, and KO5429, which accumulated lower quantities of all... Tipo: Info:eu-repo/semantics/article Palavras-chave: Glycine max; Β-conglycinin; Glycinin; Protein content; Subunit composition. Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2010000700013 Registros recuperados: 2 Primeira ... 1 ... Última

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