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| Tavares,RG; Staggemeier,R; Borges,ALP; Rodrigues,MT; Castelan,LA; Vasconcelos,J; Anschau,ME; Spalding,SM. |
| In parasitology, routine laboratory diagnosis involves conventional methods, such as optical microscopy, used for the morphological identification of parasites. Currently, molecular biology techniques are increasingly used to diagnose parasite structures in order to enhance the identification and characterization of parasites. The objective of the present study was to review the main current and new diagnostic techniques for confirmation of parasite infections, namely: polymerase chain reaction (PCR), real-time polymerase chain reaction (RT-PCR), loop-mediated isothermal amplification (LAMP), Luminex xMAP, random amplified polymorphic DNA (RAPD), amplified fragment length polymorphism (AFLP), and restriction fragment length polymorphism (RFLP), in addition... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Parasite infection; Diagnosis; Molecular techniques; Molecular epidemiology. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000300003 |
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| Tavares,RG; Trevisol,RB; Comerlato,J; Dalzochio,T; Feksa,LR; Spilki,FR; Berlese,DB. |
| Several health organizations have classified diabetes mellitus, a metabolic syndrome, as the epidemic of the century, since it affects millions of people worldwide and is one of the top ten causes of death. Type 1 diabetes is considered to be an autoimmune disease, in which autoaggressive T cells infiltrate the islets of Langerhans in the pancreas, leading to the destruction of insulin producing beta cells. The risk of the disease is modulated by genetic factors, mainly genes coding for human leukocyte antigens (HLA). However, the incidence of this disease has increased significantly during the recent decades, which cannot be explained only by genetic factors. Environmental perturbations have also been associated to the development of diabetes. Among these... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Diabetes mellitus type I; Enterovirus; Autoimmune disease. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992012000100002 |
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