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| Moraes,Paulo Ricardo de Souza; Chimara,Erica; Telles,Maria Alice da Silva; Ueki,Suely Yoko Misuka; Cunha,Eunice Atsuko Totumi; Honer,Michael Robin; Leão,Sylvia Cardoso. |
| Non-tuberculous mycobacteria isolated at the Central Public Health Laboratory from Mato Grosso do Sul in 2003 and 2004 were identified by conventional phenotypic methods (TI) and by PCR-Restriction Enzyme Analysis (PRA) using the hsp65 gene as target (PRA-hsp65). With 15 of the 32 analysed isolates, results of both methods were concordant, being 8 Mycobacterium avium, 3 M. fortutium, 1 M. kansasii, 1 M. flavescens, 1 M. peregrinum and 1 Nocardia brasiliensis. TI of 12 isolates was inconclusive. Novel PRA-hsp65 patterns were observed with 11 isolates. Medical data were evaluated for inference of clinical relevance of these isolates. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Mycobacterium; PCR; Identification. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000200013 |
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| Monteiro,Paulo Henrique Tasso; Martins,Maria Conceição; Ueki,Suely Yoko Mizuka; Giampaglia,Carmen Maria Saraiva; Telles,Maria Alice da Silva. |
| The identification of Mycobacterium tuberculosis complex (MT), using non-molecular methods, is time-consuming. The objective of this study was to evaluate a screening test for the presumptive identification of MT, which could potentially decrease laboratory turn-around time for reporting preliminary results. From January 1998 to December 1999, 3056 cultures were analysed at the Mycobacterial Laboratory, Instituto Adolfo Lutz, São Paulo, Brasil. The screening test consisted of observation of colony morphology on Löwenstein Jensen medium and evaluation of cord formation on smear microscopy from those positive cultures. After the screening test, the cultures identified as non-tuberculous mycobacteria were identified to species by conventional methods (growth... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Mycobacterium tuberculosis; Identification; Cord formation; Culture. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000200016 |
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