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Development and validation of a method for the detection of altered resistance in transgenic plants against herbivore-pathogen-complexes Nature Precedings
Nadine Drechsler; Antje Habekuß; Thomas Thieme; Jörg Schubert.
Enhanced or reduced uptake of viruses by vectors and changes in resistance level can be sensitive indicators for metabolic changes in transgenic plants caused by the new trait and not observed by conventional methods. In addition, the plant transformation process itself can lead to such changes. To be able to investigate this hypothesis in cereals we decided to use two highly important insect-transmitted viruses infecting them - _Barley yellow dwarf virus_ (BYDV) and _Wheat dwarf virus_ (WDV). Corresponding molecular tools for their quantification in plants as well as virus vectors had to be developed. 
Both viruses cause similar symptoms: dwarfing, stunting, leaf discoloration leading to yield losses or death of plant. BYDV...
Tipo: Poster Palavras-chave: Plant Biology.
Ano: 2010 URL: http://precedings.nature.com/documents/4493/version/1
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Development and validation of an analysis method for the detection of altered resistance in transgenic plants to herbivore-pathogen-complexes Nature Precedings
Anja Hühnlein; Jörg Schubert; Thomas Thieme.
Genetically modified plants (GMPs) have to pass several safety evaluations before they can be approved but identification of undesired changes in metabolism is hard to achieve due to its complexity. Such unexpected changes could be reflected by changes in virus resistance and feeding behaviour of herbivores such as aphids. Altered behaviour can be determined by so- called life table statistics where e. g. the lifetime and the reproduction rate are analysed. Such experiments are time consuming and costly. The behaviour alterations should also be detectable by changes in virus uptake und transmission. Thus, quantitative detection methods are needed in order to measure the absolute virus content in plants and in aphids. _Potato leafroll virus_ (PLRV) and...
Tipo: Poster Palavras-chave: Plant Biology.
Ano: 2010 URL: http://precedings.nature.com/documents/4473/version/1
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