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Evaluation of MALDI-TOF mass spectrometry for the competitiveness analysis of selected indigenous cowpea (Vigna unguiculata L. Walp.) Bradyrhizobium strains from Kenya Organic Eprints
Mathu Ndungu, Samuel; Messmer, Monika M.; Ziegler, Dominik; Thuita, Moses; Vanlauwe, Bernard; Frossard, Emmanuel; Thonar, Cécile.
Cowpea N2 fixation and yield can be enhanced by selecting competitive and efficient indigenous rhizobia. Strains from contrasting agro-ecologies of Kilifi and Mbeere (Kenya) were screened. Two pot experiments were established consisting of 13 Bradyrhizobium strains; experiment 1 (11 Mbeere + CBA + BK1 from Burkina Faso), experiment 2 (12 Kilifi + CBA). Symbiotic effectiveness was assessed (shoot biomass, SPAD index and N uptake). Nodule occupancy of 13 simultaneously co-inoculated strains in each experiment was analyzed by matrix-assisted laser desorption/ionization time of flight (MALDI-TOF) mass spectrometry (MS) to assess competitiveness. Strains varied in effectiveness and competitiveness. The four most efficient strains were further evaluated in a...
Tipo: Journal paper Palavras-chave: Crop health; Quality; Protection Africa.
Ano: 2018 URL: http://orgprints.org/33100/1/Ndungu-etal-2018-AMB-online-p1-14.pdf
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Identification of Heterotrophic Zinc Mobilization Processes among Bacterial Strains Isolated from Wheat Rhizosphere (Triticum aestivum L.) Organic Eprints
Costerousse, Benjamin; Schönholzer-Mauclaire, Laurie; Frossard, Emmanuel; Thonar, Cécile.
Soil and plant inoculation with heterotrophic zinc-solubilizing bacteria (ZSB) is considered a promising approach for increasing zinc (Zn) phytoavailability and enhancing crop growth and nutritional quality. Nevertheless, it is necessary to understand the underlying bacterial solubilization processes to predict their repeatability in inoculation strategies. Acidification via gluconic acid production remains the most reported process. In this study, wheat rhizosphere soil serial dilutions were plated on several solid microbiological media supplemented with scarcely soluble Zn oxide (ZnO), and 115 putative Zn-solubilizing isolates were directly detected based on the formation of solubilization halos around the colonies. Eight strains were selected based on...
Tipo: Journal paper Palavras-chave: Cereals; Pulses and oilseeds Nutrient turnover Crop health; Quality; Protection.
Ano: 2018 URL: http://orgprints.org/32662/1/Costerousse-etal-2018-ApplEnvironMicrobiol-vol84-issue1-p1-16.pdf
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Potential of three microbial bio-effectors to promote maize growth and nutrient acquisition from alternative phosphorous fertilizers in contrasting soils Organic Eprints
Thonar, Cécile; Lekfeldt, Jonas D.S.; Cozzolino, Vincenza; Kundel, Domimika; Kulhánek, Martin; Mosimann, Carla; Neumann, Günter; Piccolo, Alessandro; Rex, Martin; Symanczik, Sarah; Walder, Florian; Weinmann, Markus; de Neegaard, Andreas; Mäder, Paul.
Background Agricultural production is challenged by the limitation of non-renewable resources. Alternative fertilizers are promoted but they often have a lower availability of key macronutrients, especially phosphorus (P). Biological inoculants, the so-called bio-effectors (BEs), may be combined with these fertilizers to improve the nutrient use efficiency. Methods The goal of this study was to assess the potential of three BEs in combination with alternative fertilizers (e.g., composted manure, biogas digestate, green compost) to promote plant growth and nutrient uptake in soils typical for various European regions. Pot experiments were conducted in Czech Republic, Denmark, Germany, Italy, and Switzerland where the same variety of maize was grown in...
Tipo: Journal paper Palavras-chave: Soil quality; Crop combinations and interactions; Composting and manuring.
Ano: 2017 URL: http://orgprints.org/31321/1/Thonar_et_al-2017-Chemical_and_Biological_Technologies_in_Agriculture.pdf
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Selektion von kühletoleranten Bradyrhizobien Stämmen und Interaktion mit frühreifen Sojasorten Organic Eprints
Messmer, Monika; Berset, Estelle; Hertenstein, Florian; Peláez, Sarah; Salomé, Clémence; Thonar, Cécile; Wilbois, Klaus-Peter; Zimmer, Steffi; Haase, Thorsten; Hess, Jürgen; Habekuss, Antje; Kleemann, Michael; Ordon, Frank; Vogt-Kaute, Werner; Vogel, Rainer; Unsleber, Jürgen; Littmann, Christian.
- Arbeitshypothese - Inokulationsversuche bei unterschiedlichem Temperaturregime - Feldversuche mit verschiedenen kommerziellen Produkten - Erste Schlussfolgerungen
Tipo: Conference paper, poster, etc. Palavras-chave: Cereals; Pulses and oilseeds Soil Breeding; Genetics and propagation.
Ano: 2012 URL: http://orgprints.org/26565/1/GPZ%20Tagung%20Leguminosen%20Eckhardsweier%2012%2009%202012%20v2.pdf
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Metabolite profiling on wheat grain to enable a distinction of samples from organic and conventional farming systems Organic Eprints
Bonte, Anja; Neuweger, Heiko; Goesmann, Alexander; Thonar, Cécile; Mäder, Paul; Langenkämper, Georg; Niehaus, Karsten.
Identification of biomarkers capable of distinguishing organic and conventional products would be highly welcome to improve the strength of food quality assurance. Metabolite profiling was used for biomarker search in organic and conventional wheat grain (Triticum aestivum L.) of 11 different old and new bread wheat cultivars grown in the DOK system comparison trial. Metabolites were extracted usingmethanol and analysed by gas chromatography–mass spectrometry.
Tipo: Journal paper Palavras-chave: Cereals; Pulses and oilseeds.
Ano: 2014 URL: http://orgprints.org/25739/1/bonteetal2014.pdf
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Green manure and long-term fertilization effects on soil zinc and cadmium availability and uptake by wheat (Triticum aestivum L.) at different growth stages Organic Eprints
Grüter, Roman; Costerousse, Benjamin; Bertoni, Angelina; Mayer, Jochen; Thonar, Cécile; Frossard, Emmanuel; Schulin, Rainer; Tandy, Susan.
Zinc (Zn) deficiency in human populations depending on cereals as a main source of Zn is a global malnutrition problem. In this field study, we investigated the potential of green manure application to increase soil Zn availability and wheat grain Zn concentrations (biofortification) on a Luvisol with different long-term fertilizer management. We also studied cadmium (Cd), as wheat is a major contributor of this undesired non-essential element to human diets. Clover (Trifolium alexandrinum L.), mustard (Sinapis alba L.) or no green manure was grown on field plots which had been managed with farmyard manure or mineral fertilizers for 65 years in Switzerland. After green manure incorporation into the soil, spring wheat (Triticum aestivum L.) was grown on all...
Tipo: Journal paper Palavras-chave: Cereals; Pulses and oilseeds Composting and manuring Nutrient turnover.
Ano: 2017 URL: http://orgprints.org/32663/1/greuter-etal-2017-SciTotalEnv-vol599-600-p1330-1343.pdf
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Long term farming systems affect soils potential for N2O production and reduction processes under denitrifying conditions Organic Eprints
Krause, Hans-Martin; Thonar, Cécile; Eschenbach, Wolfram; Well, Reinhard; Mäder, Paul; Behrens, Sebastian; Kappler, Andreas; Gattinger, Andreas.
N2O is a potent greenhouse gas with an atmospheric lifetime of 114 years which also contributes to ozone layer destruction. Mitigating N2O emissions is especially challenging to the agricultural sector that is responsible for the majority of anthropogenic N2O release. In order to develop effective mitigation strategies, a detailed understanding of drivers for N2O production and reduction in agriculturally managed soils is needed. Denitrification is recognized as one of the most important source processes for N2O emissions from soils. However, the last step in denitrification, the reduction of N2O to N2 is the only known sink for N2O in soil. Although the impact of single parameters on denitrification is quite well documented, there is still a knowledge gap...
Tipo: Journal paper Palavras-chave: Soil quality; Composting and manuring; Nutrient turnover.
Ano: 2017 URL: http://orgprints.org/32664/1/krause-etal-2017-SoilBioChem-Vol114-p31-41.pdf
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Breeding for symbioses – Mycorrhizae as a case study Organic Eprints
Hohmann, Pierre; Backes, Gunter; Thonar, Cécile; Messmer, Monika.
Plant associated soil microbes are known to play an important role in the expression and stability of certain plant traits such as nutrient use efficiency and disease resistance. Arbuscular mycorrhizal fungi (AMF) form one of the primary mutualistic plant-microbe symbioses. Besides known benefits such as improved nutrient mobilisation (mainly phosphorus and zinc) and tolerance against abiotic stresses (mainly drought), an increasing number of studies highlight a significant role of AMF in the mediation of disease resistances and priming mechanisms. Individual reports have shown enhanced levels of defence-related compounds (such as glucanases, chitinases and phenolics) in mycorrhizal plants, and there is first evidence of certain phytohormone pathways (in...
Tipo: Conference paper, poster, etc. Palavras-chave: Breeding; Genetics and propagation Crop health; Quality; Protection.
Ano: 2016 URL: http://orgprints.org/30510/1/hohmann-etal-2016-Eucarpia2016_Abstract.pdf
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Selektion der Soja und deren Symbiosepartner auf Kühletoleranz und Effizienz der biologischen Stickstoff-Fixierung Organic Eprints
Berset, Estelle; Hertenstein, Florian; Peláez, Sarah; Bufe, Cornelia; Thonar, Cécile; Wilbois, Klaus-Peter; Messmer, Monika.
In Central Europe low temperature is limiting soybean growth and biological nitrogen fixation (BNF). The BNF efficiency was shown to increase with bradyrhizobia strains selected for their cold tolerance. Significant bradyrhizobia x soybean variety interactions were reported. Co-inoculation with arbuscular mycorrhiza fungi (AMF) may promote bradyrhizobia and improve plant phosphorus uptake. The aim of this study is to identify (i) bradyrhizobia showing improved nodulation under cool conditions, (ii) bradyrhizobia x soybean variety and (iii) bradyrhizobia x AMF interactions. In 2011 twelve bradyrhizobia inoculants were tested with three soybean (Glycine max [L.]) varieties under three temperature regimes (14/10°C, 16/12°C, 22/20 °C) in a pot trial. In 2012...
Tipo: Conference paper, poster, etc. Palavras-chave: Breeding; Genetics and propagation Crop health; Quality; Protection.
Ano: 2013 URL: http://orgprints.org/21470/1/21470_Berset.pdf
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Potential of three microbial bio-effectors to promote maize growth and nutrient acquisition from alternative phosphorous fertilizers in contrasting soils Organic Eprints
Thonar, Cécile; Lekfeldt, Jonas D.S.; Cozzolino, Vincenza; Kundel, Domimika; Kulhánek, Martin; Mosimann, Carla; Neumann, Günter; Piccolo, Alessandro; Rex, Martin; Symanczik, Sarah; Walder, Florian; Weinmann, Markus; de Neergaard, Andreas; Mäder, Paul.
Background: Agricultural production is challenged by the limitation of non-renewable resources. Alternative fertilizers are promoted but they often have a lower availability of key macronutrients, especially phosphorus (P). Biological inoculants, the so-called bio-effectors (BEs), may be combined with these fertilizers to improve the nutrient use efficiency. Methods: The goal of this study was to assess the potential of three BEs in combination with alternative fertilizers (e.g., composted manure, biogas digestate, green compost) to promote plant growth and nutrient uptake in soils typical for various European regions. Pot experiments were conducted in Czech Republic, Denmark, Germany, Italy, and Switzerland where the same variety of maize was grown in...
Tipo: Journal paper Palavras-chave: Composting and manuring; Nutrient turnover.
Ano: 2017 URL: http://orgprints.org/32920/1/Thonar2017-Potential%20of%20three%20microbial%20bio%E2%80%91effectors%20to%20promote%20maize%20growth%20and%20nutrient%20acquisition%20from%20alternative%20phosphorous%20fertilizers%20in%20contrasting%20soils.pdf
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Breeding for improved soybean-Bradyrhizobia symbiosis for cool growing conditions in Central Europe Organic Eprints
Messmer, Monika; Berset, Estelle; Zimmer, Steffi; Haase, Thorsten; Habekuss, Antje; Paláez, Sarah; Hertenstein, Florian; Thonar, Cécile; Salomé, Clémence; Ordon, Frank; Hess, Jürgen; Wilbois, Klaus-Peter.
Background In recent years soybean (Glycine max L.) has gained significant attention in Europe due to its ability to produce high quality protein for human and animal consumption. Under the climatic conditions prevalent in Central Europe, low temperature is the major factor limiting soybean growth and symbiotic nitrogen fixation.
Tipo: Conference paper, poster, etc. Palavras-chave: Cereals; Pulses and oilseeds Breeding; Genetics and propagation.
Ano: 2012 URL: http://orgprints.org/21797/1/Poster_BreedingSoybeaninCentralEurope__final.pdf
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Multi-purpose cowpea inoculation for improved yields in small holder farms in Kenya Organic Eprints
Mathu, Samuel; Thonar, Cécile; Vanlauwe, Bernard; Messmer, Monika; Frossard, Emmanuel.
Introduction In Kenya, cowpea is the most important pulse crop in the dry lands of Eastern and Coastal regions where it is commonly inter cropped with maize and sorghum. The poor yields obtained in small holder farms in Kenya (150 kg ha-1) can in part be attributed to the use of poor yielding varieties, low soil fertility (mainly N and P deficiency) low presence of effective indigenous rhizobia and high occurrence of highly competitive but inefficient indigenous rhizobia strains. Biological nitrogen fixation (BNF) through exploitation of the rhizobia-legume symbiosis and use of inoculants coupled with soil amendments such as Phosphorus offers in part a means to improve cowpea yield, nutrition and soil fertility.
Tipo: Conference paper, poster, etc. Palavras-chave: Soil quality Breeding; Genetics and propagation Africa.
Ano: 2012 URL: http://orgprints.org/22169/1/mathu-etal-2012-Poster-ISFM-nairobi-kenya.pdf
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Einsatz von Protein- und Metabolit-Profiling-Methoden zur Unterscheidung von ökologischem und konventionellem Weizen Organic Eprints
Bonte, Anja; Kessler, Nikolas; Nattkemper, Tim; Goesmann, Alexander; Thonar, Cécile; Mäder, Paul; Niehaus, Karsten; Langenkämper, Georg.
The interest in methods to proof organic food authenticity increases with the steadily rising popularity of food labelled organic. Profiling techniques enable the detection of a wide range of substances in biological samples. Together with bioinformatics tools these techniques are useful for biomarker searching, e. g. in plant extracts. Metabolomic and proteomic profiling techniques were used to screen organic and conventional wheat, originating from the DOK field trial in Switzerland. Up to 11 wheat varieties from three harvest years were analysed. We were able to detect a number of metabolites and proteins with significant differences between samples of conventional and organic grown wheat of the variety “Runal”. Results viewed across all 11 varieties...
Tipo: Conference paper, poster, etc. Palavras-chave: Indicators and other value-laden measures Food security; Food quality and human health.
Ano: 2013 URL: http://orgprints.org/21336/1/21336_langenk%C3%A4mper.pdf
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Tracing of Two Pseudomonas Strains in the Rootand Rhizoplane of Maize, as Related to Their Plant Growth-Promoting Effectin Contrasting Soils Organic Eprints
Mosimann, Carla; Oberhänsli, Thomas; Ziegler, Dominik; Nassal, Dinah; Kandeler, Ellen; Boller, Thomas; Mäder, Paul; Thonar, Cécile.
TaqMan-based quantitative PCR (qPCR) assays were developed to study the persistence of two well-characterized strains of plant growth-promoting rhizobacteria (PGPR), Pseudomonas fluorescens Pf153 and Pseudomonas sp. DSMZ 13134, in the root and rhizoplane of inoculated maize plants. This was performed in pot experiments with three contrasting field soils (Buus, Le Caron and DOK-M). Potential cross-reactivity of the qPCR assays was assessed with indigenous Pseudomonas and related bacterial species, which had been isolated from the rhizoplane of maize roots grown in the three soils and then characterized by Matrix-Assisted Laser Desorption Ionization (MALDI) Time-of-Flight (TOF) mass spectrometry (MS). Sensitivity of the qPCR expressed as detection limit of...
Tipo: Journal paper Palavras-chave: Soil biology Crop health; Quality; Protection.
Ano: 2017 URL: http://orgprints.org/31294/1/mosimann-etal-2017-fmicb-vol7-article02150.pdf
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Application of Mycorrhiza and Soil from a Permaculture System Improved Phosphorus Acquisition in Naranjilla Organic Eprints
Symanczik, Sarah; Gisler, Michelle; Thonar, Cécile; Schlaeppi, Klaus; van der Heijden, Marcel; Kahmen, Ansgar; Boller, Thomas; Mäder, Paul.
Naranjilla (Solanum quitoense) is a perennial shrub plant mainly cultivated in Ecuador, Colombia, and Central America where it represents an important cash crop. Current cultivation practices not only cause deforestation and large-scale soil degradation but also make plants highly susceptible to pests and diseases. The use of arbuscular mycorrhizal fungi (AMF) can offer a possibility to overcome these problems. AMF can act beneficially in various ways, for example by improving plant nutrition and growth, water relations, soil structure and stability and protection against biotic and abiotic stresses. In this study, the impact of AMF inoculation on growth and nutrition parameters of naranjilla has been assessed. For inoculation three European reference AMF...
Tipo: Journal paper Palavras-chave: Soil Crop health; Quality; Protection.
Ano: 2017 URL: http://orgprints.org/33353/1/Symanczik-etal-2017_FrontPlantSci_Vol8_Article1263_pp1-12.pdf
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Cowpea (Vigna unguiculata L. Walp) hosts several widespread bradyrhizobial root nodule symbionts across contrasting agro-ecological production areas in Kenya Organic Eprints
Mathu Ndungu, Samuel; Messmer, Monika; Ziegler, Dominik; Gamper, Hannes; Mészáros, Éva; Thuita, Moses; Vanlauwe, Bernard; Frossard, Emmanuel; Thonar, Cécile.
Cowpea (Vigna unguiculata L. Walp.) is an important African food legume suitable for dry regions. It is the main legume in two contrasting agro-ecological regions of Kenya as an important component of crop rotations because of its relative tolerance to unpredictable drought events. This study was carried out in an effort to establish a collection of bacterial root nodule symbionts and determine their relationship to physicochemical soil parameters as well as any geographical distributional patterns. Bradyrhizobium spp. were found to be widespread in this study and several different types could be identified at each site. Unique but rare symbionts were recovered from the nodules of plants sampled in a drier in-land region, where there were also overall more...
Tipo: Journal paper Palavras-chave: Vegetables Crop health; Quality; Protection Africa.
Ano: 2018 URL: http://orgprints.org/33099/1/Ndungu-etal-2018-AEE-Vol261-p161-171.pdf
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Long-term organic matter application reduces cadmium but not zinc concentrations in wheat Organic Eprints
Grüter, Roman; Costerousse, Benjamin; Mayer, Jochen; Mäder, Paul; Thonar, Cécile; Frossard, Emmanuel; Schulin, Rainer; Tandy, Susan.
Wheat is a staple food crop and a major source of both the essential micronutrient zinc (Zn) and the toxic heavy metal cadmium (Cd) for humans. Since Zn and Cd are chemically similar, increasing Zn concentrations in wheat grains (biofortification), while preventing Cd accumulation, is an agronomic challenge. We used two Swiss agricultural long-term field trials, the “Dynamic-Organic-Conventional System Comparison Trial” (DOK) and the “Zurich Organic Fertilization Experiment” (ZOFE), to investigate the impact of long-term organic, mineral and combined fertilizer inputs on total and phytoavailable concentrations of soil Zn and Cd and their accumulation in winter wheat (Triticum aestivum L.). “Diffusive gradients in thin films” (DGT) and...
Tipo: Journal paper Palavras-chave: Soil quality Cereals; Pulses and oilseeds Composting and manuring.
Ano: 2019 URL: http://orgprints.org/36388/1/grueter-etal-2019-SciTotalEnv-Vol669-p608-620.pdf
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Organic Knowledge Network Arable - D.3.1 State-of-the-art research results and best practices Organic Eprints
Niggli, Urs; Schmidt, Jennifer; Watson, Christine; Kriipsalu, Mait; Shanskiy, Merrit; Barberi, Paolo; Kowalska, Jolanta; Schmitt, Annegret; Daniel, Claudia; Wenthe, Ulla; Conder, Malgorzarta; Wohlfahrt, Jens; Schild, Marion; Dierauer, Hansueli; Krauss, Maike; Moeskops, Bram; Padel, Susanne; Micheloni, Cristina; Constanzo, Ambrogio; Thonar, Cécile; Wilbois, Klaus-Peter.
In this deliverable of the Project OK-Net Arable project, the findings from peer-review research on the productivity of organic arable crops are reported. Then the conclusions and recommendations for five most important levers which can be used by the farmers are presented. Finally, the most important recommendations of the EIP-AGRI Focus Group on Organic Farming - Optimizing Arable Yields are summarized in order to put innovation on organic farms in its social context.
Tipo: Report Palavras-chave: Crop husbandry; Soil.
Ano: 2016 URL: http://orgprints.org/30506/1/OK_Net_WP3_D31_final.pdf
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Real-time PCR to quantify composition of arbuscular mycorrhizal fungal communities—marker design, verification, calibration and field validation Organic Eprints
Thonar, Cécile; Erb, A.; Jansa, J..
Quantitative real-time PCR (qPCR) is slowly becoming established as a tool to quantify abundance of different arbuscular mycorrhizal fungal (AMF) taxa in roots and in soil. Here, we describe the development and field validation of qPCR markers (i.e. primers with associated hydrolysis probes), targeting taxon-specific motifs in the nuclear large ribosomal subunit RNA genes. Design of such markers is complicated by the multinuclear and multigenomic cellular organization of these fungi and the high DNA sequence diversity within the smallest biologically relevant units (i.e. single-spore isolates). These limitations are further compounded by inefficient biomass production of these fungi, resulting in limited availability of pure genomic DNA (gDNA) of...
Tipo: Journal paper Palavras-chave: Soil.
Ano: 2011 URL: http://orgprints.org/25988/1/Thonar_et_al_2011_CT33.pdf
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