|
|
|
|
|
| Titze-de-Almeida,Ricardo; Rollo Filho,Maurício; Nogueira,Celeste A.; Rodrigues,Isabela P.; Eudes Filho,João; Nascimento,Rejane S. do; Ferreira II,Renato F.; Moraes,Lídia M. P.; Boelens,Hélène; Van Belkum,Alex; Felipe,Maria Sueli Soares. |
| We studied the antimicrobial resistance and the molecular epidemiology of 99 enterococcal surveillance isolates from two hospitals of Brasília, Brazil. Conventional biochemical tests were used to identify the enterococcal species and the disk diffusion method was used to determine their resistance profiles. Enterococcus faecalis (76%) and E. faecium (9%) were the most prevalent species. No enterococci showed the vanA or vanB vancomycin resistance phenotypes or genotypes. Only the intrinsically resistant species E. gallinarum (n=2) and E. casseliflavus (n=3) harbored the vancomycin-resistance genes vanC1 and vanC2/3, respectively. We found E. faecalis isolates with high-level resistance to gentamicin (22%) and streptomycin (8%) and both E. faecalis and E.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Enterococci; Antimicrobial resistance; Genotyping. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702004000300002 |
| |
|
|
| d'Azevedo,Pedro Alves; Santiago,Kelly Aline de Souza; Furtado,Guilherme Henrique Campos; Xavier,Diego Batista; Pignatari,Antonio Carlos Campos; Titze-de-Almeida,Ricardo. |
| The reduction in time required to identify vancomycin-resistant enterococci (VRE) has gained increased importance during hospital outbreaks. In the present study, we implemented a laboratory protocol to speed up the VRE screening from rectal samples. The protocol combines a medium for selective VRE isolation (VREBAC®, Probac, São Paulo) and a multiplex PCR for detection and identification of vanA and vanB resistance genes. The screening performance was analyzed in 114 specimens collected from four intensive care units. The swabs were collected at two periods: (1) during a VRE outbreak (February 2006, n=83 patients) and (2) at the post-outbreak period, after adoption of infection control measures (June 2006, n=31 patients). Forty-one/83 VRE (49.4%) and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Enterococcus; VRE; Vancomycin; Diagnostic; PCR. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702009000400010 |
| |
|
|
|
