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| Ohmori, Takashi; Tamahara, Satoshi; Thi, Pham Ngoc; Tomihari, Mizuki; Shimada, Terumasa; Matsuki, Naoaki; Ono, Kenichiro. |
| Interleukin-12 (IL-12) is one of the most important factors for the host’s protective immune response against protozoan infection. To evaluate the effect of bioactive form of IL-12(IL-12p70), expression of IFN-AmRNA and IL-4 mRNA in splenic CD4 positive T cells, anti-parasite delayed type hypersensitivity (DTH), and serum and splenic IL-12p70 concentration were examined in Babesia microti and B. rodhaini infected mice. The expression of IFN-AmRNA as a marker of Th1 cell differentiation was detected in splenic CD4 positive T cells in B. microti infected mice. Activation of DTH response as a marker of cell-mediated immune response was also observed from 2 days after the infection with B. microti, while no response was detected in B. rodhaini infected mice.... |
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Palavras-chave: Babesia microti; Babesia rodhaini; Cell-mediated immunity; Early phase of infection; IL-12p70; Th1 cell differentiation. |
| Ano: 2005 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/150 |
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| Tagawa, Michihito; Yamamoto, Yuhei; Shimbo, Genya; Iguchi, Aiko; Xuan, Xuenan; Tomihari, Mizuki; Miyahara, Kazuro. |
| Cytotoxic T lymphocyte associated gene-4 (CTLA-4) is a costimulatory molecule, expressed on the surface of activated T cells that negatively regulates T cell activation. In humans, alternative splicing of the CTLA-4 gene generates two major isoforms of mRNA, and a soluble form of CTLA-4 (sCTLA-4) was detected in normal human serum. We describe alternatively spliced mRNA expressed in peripheral blood mononuclear cells obtained from a healthy dog lacking the transmembrane domain coded by exon 3 of the CTLA-4 gene. Immunoprecipitation and western blotting of dog serum revealed a band of approximately 23-kDa, which is consistent with the predicted size, based on the amino acid sequence of the canine sCTLA-4 obtained in this study. |
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Palavras-chave: CD152; CTLA-4; Dog; Soluble form; Splicing variant. |
| Ano: 2017 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4517 |
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