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| Zeni,Jamile; Gomes,Jonaína; Ambroszini,Éllin; Basso,Ana Paula; Toniazzo,Geciane; Valduga,Eunice. |
| Penicillium brasilianum was previously isolated from tea and identified by molecular biology technique. A Plackett-Burman design, followed by a complete second order design was used for the screening of most important factors and to maximize the pectin liase (PMGL) activity, respectively. The maximum PMGL activity by P. brasilianum achieved was 9.0 U/mL after 48 h of cultivation in a medium containing pectin (33.0 g/L), yeast extract (30.0 g/L) and potassium phosphate (2.0 g/L) at 30ºC, with a stirring rate of 180 rpm, initial pH 5.5 and 5x106spores/mL inoculum size. The kinetic evaluation in terms of substrate consumption demonstrated that the maximum production of PMGL was at 72 h, and 40% of the total organic carbon, 25% of the nitrogen, 88% of the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Penicillium brasilianum; Pectin liase; Experimental design technique. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000600908 |
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| Brustolin,Jean Carlos; Dal Pisol,Andreia; Steffens,Juliana; Toniazzo,Geciane; Valduga,Eunice; Di Luccio,Marco; Cansian,Rogério Luis. |
| The objective of this work was to evaluate the effect of different water pressures and concentrations of lactic acid on microbial counts (mesophilic bacteria, enterobacteria and Salmonella) on pig carcasses without contamination and contaminated carcasses, before and after the last shower and before being cooled. The tests were carried out using 4, 3 and 2 bar water pressure, and 2, 1 and 0% lactic acid concentration. In general, both the pressure in the shower and lactic acid had a positive effect by reducing the microbial count. The interaction between the pressure and lactic acid caused the largest reduction in carcasses surface count for mesophiles. With regard to enterobacteria on contaminated carcasses, the most important variable was the lactic acid... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Pig carcasses; Decontamination; Water pressure; Lactic acid; Bacteria. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000600954 |
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| Lopes,Marcos; Paroul,Natalia; Barbosa,Juliana; Valduga,Eunice; Cansian,Rogerio Luis; Toniazzo,Geciane; Oliveira,Débora. |
| ABSTRACT Among the nutrients involved in the chicken diet, the microminerals deserve attention as they exert essential functions in the organism. These compounds can be provided in inorganic (traditional) and organic (chelate) forms. In organic form, the micromimerals can attend a new concept related to a better bio-availability. In this sense, the objective of this work was to evaluate the effect of the partial and total substitution of inorganic by organic sources of microminerals on the animal diet, assessing its concentration in the blood and liver after 21 and 40 days and also in the bones after 40 days. Moreover, the effect on the physical-chemical and sensory characteristics of the muscle after 15 days of storage at 4°C was also evaluated. The... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bio-availability; Broilers; Microminerals; Nutrition. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100440 |
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| Beltrame,Cezar Augusto; Kubiak,Gabriela Busnello; Lerin,Lindomar Alberto; Rottava,Ieda; Mossi,Altemir José; Oliveira,Débora de; Cansian,Rogério Luis; Treichel,Helen; Toniazzo,Geciane. |
| The efficiency of four Sanitizers - peracetic acid, chlorhexidine, quaternary ammonium, and organic acids - was tested in this work using different bacteria recognized as a problem to meat industry, Salmonella sp., S. aureus, E. coli and L. monocytogenes. The effects of sanitizer concentration (0.2, 0.5, 0.6, 1.0, 1.1 and 1.4%), at different temperatures (10 and 45 °C) and contact time (2, 10, 15, 18 and 25 minutes) were evaluated. Tests in an industrial plant were also carried out considering previously obtained results. In a general way, peracetic acid presented higher efficiencies using low concentration (0.2%) and contact time (2 minutes) at 10 °C. The tests performed in industrial scale showed that peracetic acid presented a good performance in... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Sanitizers; Bacteria; Food industries. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612012000200003 |
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| Lerin,Lindomar; Toniazzo,Geciane; Oliveira,Débora de; Rottava,Leda; Dariva,Cláudio; Cansian,Rogério Luis; Treichel,Helen; Padilha,Francine; Antunes,Octávio Augusto Ceva. |
| Limonene is a monoterpene obtained in large amounts from essential oils and is used as a raw material for the synthesis of flavors and fine chemicals. Several pathways or routes for the microbial degradation of limonene making use of the cytochrome P450-dependent monooxygenases have been described. In this study, we present a fermentative screening of microorganisms in order to verify their ability to perform the desirable conversion. In parallel, the PCR technique was used to select the microorganisms that contain the limC gene, which is responsible for the conversion of carveol to carvone. The microorganisms selected by PCR were not able to bioconvert limonene. From this result, we can suppose that these strains do not have the gene that codifies the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: CDH (carveol dependent dehydrogenase); Carveol; Carvone; PCR; Fermentative screening. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612010000200017 |
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| Flach,Juliana; Grzybowski,Valeska; Toniazzo,Geciane; Corção,Gertrudes. |
| Biofilms in milk cooling tanks compromise product quality even on farms. Due to the lack of studies of this topic, this study evaluated the microbiological conditions of raw milk cooling tanks on farms and characterized the microorganisms isolated from these tanks. Samples were wiped off with sterile swabs from seven milk cooling tanks in three different points in each tank. Mesophiles and psychrotrophic counts were performed in all samples. The isolation of Pseudomonas spp., Bacillus cereus and atypical colonies formed on selective media were also performed, totalizing 297 isolates. All isolates were tested for protease and lipase production and biofilm formation. Of the total isolates, 62.9% produced protease, 55.9% produced lipase, and 50.2% produced... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Psychrotrophic bacteria; Cleaning process; Lipase; Protease. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612014000300020 |
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| Cansian,Rogério Luis; Mossi,Altemir José; Oliveira,Débora de; Toniazzo,Geciane; Treichel,Helen; Paroul,Natalia; Astolfi,Viviane; Serafini,Luciana Atti. |
| Este trabalho teve como objetivo determinar a atividade antimicrobiana e antioxidante do óleo essencial de Ho-Sho. O principal componente do óleo essencial obtido a partir de folhas da planta submetidas ao processo de hidrodestilação foi o linalol (80 a 95% m/m). O óleo essencial mostrou atividade antimicrobiana para todos os microrganismos testados, com exceção de Pseudomonas aeruginosa. A maior atividade antimicrobiana do óleo essencial sobre as bactérias testadas foi observada sobre Xanthomonas campestris (33,0 mm) e a menor sobre Yersinia enterocolitica (10,5 mm). Para a concentração inibitória mínima (CIM), observou-se que todos os microrganismos apresentaram-se susceptíveis ao óleo essencial de Ho-Sho. A variação das CIM para as bactérias... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Atividade antimicrobiana; Atividade antioxidante; Cinnamomum camphora. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612010000200014 |
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| Esmelindro,Maria Carolina; Toniazzo,Geciane; Waczuk,Adroaldo; Dariva,Cláudio; Oliveira,Débora de. |
| A erva-mate é uma matéria-prima de grande importância para a região Sul do Brasil, sendo que a produção anual é de aproximadamente 650.000 toneladas de folhas. Atualmente, problemas com o excesso de oferta têm incentivado pesquisadores e empresários a buscar alternativas para a utilização da erva-mate como matéria-prima para o desenvolvimento de novos produtos bem como promover melhorias no processamento industrial visando a obtenção de características organolépticas desejáveis. Neste sentido, o presente trabalho teve por objetivo realizar a caracterização físico-química da erva-mate em função das etapas do processamento industrial (sapeco, secagem e tempo de cancheamento) e verificar como estas etapas influem nos teores de cinzas, fibras, gorduras,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Erva-mate; Processamento industrial; Caracterização físico-química. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612002000200016 |
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| Tomicki,Letícia; Rigo,Aline Andressa; Durigon,Angelise; Gutkoski,Luiz Carlos; Zeni,Jamile; Valduga,Eunice; Steffens,Clarice; Toniazzo,Geciane. |
| Com a intenção de minimizar a carência de produtos isentos de glúten e tendo em vista o crescente aumento de celíacos, a elaboração de produtos diferenciais a base de farinha de arroz e milho torna-se uma alternativa de consumo e comercialização. Assim, este trabalho teve como objetivo avaliar os efeitos da adição de farinha de arroz e milho, e emulsificante nas características de qualidade (teste de cozimento, textura instrumental - firmeza e aceitabilidade) e físico-químicas (proteínas, lipídios, carboidratos, minerais e fibra alimentar) de formulações de macarrão, mediante o emprego de metodologia de planejamento de experimentos, fixando a concentração de ovos in natura (12%, m/m) e água (20%, v/m). A formulação com 40g de farinha de milho, 60g de... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Arroz; Milho; Massa; Celíacos.. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782015000701311 |
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