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	Provedor de dados Neotropical Entomology (2) Autor Brancalhão,Rose M.C. (2) Torquato,Ednéia F.B. (2) Franco,Valdeni S. (1) Miranda Neto,Marcilio H. de (1) Miranda Neto,Marcílio H. de (1) Palavra-chave Baculoviridae (1) Baculovirus (1) BmMNPV (1) Bombyx mori (1) Insect (1) Neural lamella (1) Perineurium (1) Polyhedra (1) Silkworm (1) Truncated octahedron (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2006 (2) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Nucleopolyhedrovirus: scanning electron microscopy technique Neotropical Entomology Torquato,Ednéia F.B.; Miranda Neto,Marcílio H. de; Brancalhão,Rose M.C.; Franco,Valdeni S.. A simplified methodology was developed to study the geometric form of multiple Bombyx mori Nucleopolyhedrovirus by scanning electron microscopy. The virus belongs to Baculoviridae family and was isolated from the silkworm Bombyx mori (L.) (Lepidoptera: Bombycidae). The polyhedra of Nucleopolyhedrovirus were obtained from the filtrate, inoculum and hemolymph of the silkworm experimentally infected with nuclear polyhedra. This material was placed on stubs, where a copper tape was previously adhered. After dry at room temperature the virus was covered with carbon and gold. Scanning electron microscopy analysis reveled a well defined morphology for the polyhedra of multiple Bombyx mori Nucleopolyhedrovirus, making possible the mathematical study that... Tipo: Info:eu-repo/semantics/article Palavras-chave: Baculovirus; Bombyx mori; BmMNPV; Polyhedra; Truncated octahedron. Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2006000600011 Nucleopolyhedrovirus infected central nervous system cells of Bombyx mori (L.) (Lepidoptera: Bombycidae) Neotropical Entomology Torquato,Ednéia F.B.; Miranda Neto,Marcilio H. de; Brancalhão,Rose M.C.. BmMNPV, a Nucleopolyhedrovirus isolated from infected Bombyx mori (L.) larvae in Paraná State - Brazil, was used to inoculate healthy 5th-instar B. mori larvae and examine the infection on central nervous system (CNS) cells. Samples of nervous tissue were removed from the infected insects, at different sampling times, and processed for cytopathology studies by light and transmission electron microscopy using routine techniques. The experiment included both inoculated and non-inoculated larvae (control). BmMNPV infection was detected on the 5th day after inoculation in CNS cells. Initially, infection was characterized by nuclear hypertrophy and the presence of virogenic stroma, in which the progeny virions were produced. Virions are enveloped and occluded... Tipo: Info:eu-repo/semantics/article Palavras-chave: Insect; Silkworm; Baculoviridae; Neural lamella; Perineurium. Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2006000100010 Registros recuperados: 2 Primeira ... 1 ... Última

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