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| Romano,Eduardo; Ferreira,Adriana T.; Dusi,André N.; Proite,Karina; Buso,Jose A.; Ávila,Antonio C.; Nishijima,Marta L.; Nascimento,Adriana S.; Bravo-Almonacid,Fernando; Mentaberry,Alejandro; Monte,Damares; Campos,Magnólia A.; Melo,Paulo Eduardo; Cattony,Monica K.; Torres,Antonio C.. |
| The coat protein (CP) gene of the potato virus Y strain "o" (PVY O) was introduced into potato, cultivar Achat, via Agrobacterium tumefaciens-mediated transformation. Sixty three putative transgenic lines were challenged against the Brazilian strains PVY-OBR and PVY-NBR. An extremely resistant phenotype, against the two strains, was observed in one line, denominated 1P. No symptoms or positive ELISA results were observed in 16 challenged plants from this line. Another clone, named as 63P, showed a lower level of resistance. Southern blot analysis showed five copies of the CP gene in the extremely resistant line and at least three copies in the other resistant line. The stability of the integrated transgenes in the extreme resistant line was examined during... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Solanum tuberosum; GMO. |
| Ano: 2001 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362001000200004 |
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