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| Luvielmo,Márcia de Mello; Borges,Caroline Dellinghausen; Toyama,Daniela de Oliveira; Vendruscolo,Claire Tondo; Scamparini,Adilma Regina Pippa. |
| Abstract The effect of alkali stress on the yield, viscosity, gum structure, and cell ultrastructure of xanthan gum was evaluated at the end of fermentation process of xanthan production by Xanthomonas campestris pv. manihotis 280-95. Although greater xanthan production was observed after a 24 h-alkali stress process, a lower viscosity was observed when compared to the alkali stress-free gum, regardless of the alkali stress time. However, this outcome is not conclusive as further studies on gum purification are required to remove excess sodium, verify the efficiency loss and the consequent increase in the polymer viscosity. Alkali stress altered the structure of xanthan gum from a polygon-like shape to a star-like form. At the end of the fermentation,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Xanthomonas campestris; Xanthan production; Alkali stress; Transmission microscopy; Scanning electron microscopy. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000100102 |
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| Haddad Junior,Vidal; Zara,Fernando; Marangoni,Sergio; Toyama,Daniela de Oliveira; Souza,Alex Jardelino Felizardo de; Oliveira,Simone Cristina Buzzo de; Toyama,Marcos Hikari. |
| Background: Although the hydrozoan Olindias sambaquiensis is the most common jellyfish associated with human envenomation in southeastern and southern Brazil, information about the composition of its venom is rare. Thus, the present study aimed to analyze pharmacological aspects ofO. sambaquiensis venom as well as clinical manifestations observed in affected patients. Crude protein extracts were prepared from the tentacles of animals; peptides and proteins were sequenced and submitted to circular dichroism spectroscopy. Creatine kinase, cytotoxicity and hemolytic activity were evaluated by specific methods.Results: We identified two novel cytolysins denominated oshem 1 and oshem 2 from the tentacles of this jellyfish. The cytolysins presented the amino... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Olindias sambaquiensis; Hemolytic; Myonecrosis; Cytotoxicity; Cytolysin; Cnidaria venom. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992014000200316 |
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