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| Traugott, Michael; Zangerl, Petra; Juen, Anita; Schallhart, Nikolaus; Pfiffner, Lukas. |
| The use of diagnostic polymerase chain reaction (PCR) is a valuable approach to study host–parasitoid interactions. It circumvents problems inherent to rearing parasitoids from Weld-collected hosts or host dissection. Here, we present a PCR-based detection method for Diadegma semiclausum, Cotesia glomerata, and Cotesia rubecula, which are key parasitoids of the three lepidopteran cabbage pests Plutella xylostella, Pieris brassicae, and Pieris rapae, respectively. Primer pairs for the three parasitoid species and Pl. xylostella were developed: they were used either separately in singleplex PCR or combined in multiplex PCR to (1) screen simultaneously for the two Cotesia species or to (2) detect the parasitoid D. semiclausum and identify its host Pl.... |
| Tipo: Journal paper |
Palavras-chave: Crop health; Quality; Protection. |
| Ano: 2006 |
URL: http://orgprints.org/13143/1/Traugott%2Detal%2D2006%2Dbiocontrol%2Dbiol%2DControl.pdf.pdf |
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| Pfiffner, Lukas; Luka, Henryk; Schlatter, Christian; Juen, Anita; Traugott, Michael. |
| In a 2-year experiment we investigated whether wildflower strips can be used to enhance the control of cabbage moth, Mamestra brassicae L., and cabbage white butterfly, Pieris rapae L. At two sites, including six organically cultivated fields, M. brassicae egg parasitism and predation rates were determined along with an assessment of larval parasitism rates in M. brassicae and P. rapae using a DNA-based approach. Within each field, plots with and without wildflower strips were sampled and a grid design of 3 m x 3 m was used to analyse the spatial pattern of parasitism. The provision of wildflower strips provided an idiosyncratic effect on the control of lepidopterans: parasitism rates in M. brassicae eggs and larvae were not affected, whereas parasitism... |
| Tipo: Journal paper |
Palavras-chave: Biodiversity and ecosystem services Crop health; Quality; Protection. |
| Ano: 2009 |
URL: http://orgprints.org/17813/1/pfiffner-etal-2009-agriculture-ecosystems-environment.pdf |
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| Luka, Henryk; Leimgruber, Andrea; Willareth, Martin; Nagel, Peter; Pfiffner, Lukas; Wyss, Eric; Schlatter, Christian; Schied, Johannes; Traugott, Michael. |
| Beneficials can prevent or reduce outbreaks of pest species. To strengthen the populations of beneficials, their food sources must be improved in cultivated areas. The effects of habitat management for augmenting beneficial population on parasitism and predation rates of three lepidopteran pest species were investigated on two organic fields in Alten (Switzerland). The aim was to improve pest regulation through wild flower strips along fields and by planting Centaurea cyanus as companion plant within the crop. Strips of Centaurea cyanus and Fagopyrum esculentum increased parasitsm on the lepidopteran pests. C. cyanus planted as companion plant furthermore increased predation on the eggs and parasitism of the larvae of Mamestra brassicae. Several... |
| Tipo: Conference paper, poster, etc. |
Palavras-chave: Crop health; Quality; Protection. |
| Ano: 2009 |
URL: http://orgprints.org/14127/1/Luka_14127.pdf |
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