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Optimization of in vitro organogenesis in passion fruit (Passiflora edulis f. flavicarpa) Scientia Agricola
Trevisan,Flavio; Mendes,Beatriz Madalena Januzzi.
In vitro organogenesis of passion fruit was studied by the induction of adventitious buds from leaf discs in culture media supplemented with benzyladenine (BAP) or thidiazuron (TDZ). To minimize adverse effects of ethylene accumulation on shoot development, silver nitrate (AgNO3) was added to the induction media. Both BAP (0; 2.2; 4.4; 6.6 µmol L-1) and TDZ (0; 1.1; 2.2; 3.4 µmol L-1) were effective in promoting shoot development. Although no significant differences were detected using AgNO3 (23.5 µmol L-1), buds grown in AgNO3-supplemented media were more vigorous. The number of explants with buds obtained using TDZ and AgNO3-supplemented media (5.6) were higher than those obtained using BAP and AgNO3 (3.0). MSM + giberrellic acid (GA3), MSM + coconut...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Ethylene; Silver nitrate; Thidiazuron; Tissue culture.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162005000400007
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